PuSH - Publikationsserver des Helmholtz Zentrums München: Guanosine-rich oligodeoxynucleotides induce proliferation of macrophage progenitors in cultures of murine bone marrow cells.

Informationen

Hinweise zu Qualitätskriterien von Zeitschriften

Open-Access-Richtlinie der Helmholtz-Gemeinschaft 2016

CC Licencences

Metriken für Publikationen

Navigation

Startseite

English

EVA: Veröffentlichen

Neuer EVA Antrag

Recherche

Erweiterte Suche

Durchblättern nach ...

... HMGU-Autoren/Konsortien

... Organisationsstruktur

... Zeitschriften

... Publikationstypen

... Forschungsdaten

... Arbeitsgruppen

... Erscheinungsjahr

Publikationen im Überblick

Statistik

HGF Fortschrittsbericht

OA Publikationen

Eintragen

Neue Publikation eintragen

Neue Publikation holen aus...

...EVA

Fehlende Publikation melden

Highlights

Suche

Hilfe & Kontakt

Ansprechpartner

Hilfe

Datenschutz

Helmholtz Open Science

Bibliometrische Indikatoren

SHERPA/RoMEO

DOAJ

Export:

Text

Endnote (RIS) BIB

BibTeX

Lang, R.* ; Hültner, L. ; Lipford, G.B.* ; Wagner, H.* ; Heeg, K.*

Guanosine-rich oligodeoxynucleotides induce proliferation of macrophage progenitors in cultures of murine bone marrow cells.

Eur. J. Immunol. 29, 3496-3506 (1999)

Verlagsversion

DOI

	Closed

Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

Abstract
Metriken
Zusatzinfos

Widely used to specifically inhibit gene expression, synthetic oligodeoxynucleotides (ODN)can exert a plethora of non-antisense effects. Immunostimulation by CpG-ODN hasattracted particular attention. ODN rich in the nucleotide guanosine (G-rich ODN) constituteanother type of sequences displaying non-antisense-mediated effects. We have examinedthe effects of CpG- and G-rich ODN on primary mouse bone marrow cells (BMC) in vitro.CpG-ODN induced rapid proliferation of B cells and production of IL-6 and IL-12p40. How-ever, when tested in agar colony assays, CpG-ODN failed to promote the formation of colo-nies. In marked contrast, G-rich non-CpG-ODN led to sustained proliferation ofmacrophage-like cells without inducing cytokines or hemopoietic growth factors. Unlike CpG-ODN, G-rich ODN effectively induced the formation of macrophage colonies in agarassays, indicating a direct action on progenitor cells. Electrophoretic mobility shift assaysrevealed specific binding of G-rich ODN to a non-nuclear protein. The ability of a panel of ODN to compete for binding correlated with their potential to induce proliferation ofmacrophage-like cells from primary mouse BMC. As such, these data reveal a so far unrec-ognized potential of G-rich ODN to signal directly outgrowth of macrophage progenitorsfrom BMC.

Impact Factor

Scopus SNIP

Altmetric

0.000