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Kummermehr, J.C. ; Malinen, E.* ; Freykowski, S. ; Sund, M. ; Trott, K.-R.*

The influence of autologous tumor fibroblasts on the radiosensitivity of squamous cell carcinoma megacolonies.

Int. J. Radiat. Oncol. Biol. Phys. 50, 229-237 (2001)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Purpose: To study the influence of tumor fibroblasts on radiosensitivity and stem cell fraction of tumor cells in squamous cell carcinoma megacolonies by determining colony cure and clonogen survival, Methods and Materials: Murine squamous cell carcinoma cells (AT478c) grown as flat but multilayered megacolonies were co-cultured with pre-irradiated tumor fibroblasts derived from the same carcinoma, and irradiated with 1, 2, 4, or 8 fractions. Recurrent clones and their growth pattern in situ were recorded. From megacolony cure data and clonogen survival data, the clonogen number and the parameters of cellular radiosensitivity were calculated, Results: The curability of the co-cultured megacolonies, as determined by TCD50 values, was significantly increased compared to the megacolonies without fibroblasts (p < 0.01). Both the megacolony cure and clonogen survival data suggested a decrease of the clonogen fraction in the so-cultured megacolonies, Conclusion: The presence of tumor fibroblasts increases megacolony radiosensitivity. This is due to a decrease in the fraction of clonogens in the tumor megacolony, apparently caused by a downregulation of the stem cell fraction of the tumor cells.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter squamous cell carcinoma cells; megacolony system; tumor cell fibroblast interaction; fractionation response; radiosensitivity; IN-VITRO; GROWTH; CULTURES; STROMA
Sprache englisch
Veröffentlichungsjahr 2001
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0360-3016
e-ISSN 0360-3016
Zeitschrift International Journal of Radiation Oncology, Biology, Physics
Quellenangaben Band: 50, Heft: 1, Seiten: 229-237 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)
Department for Medical Information Systems (MEDIS)
Institute of Health Economics and Health Care Management (IGM)
PubMed ID 11316568
DOI 10.1016/S0360-3016(01)01454-7
WOS ID WOS:000168316200029
Erfassungsdatum 2002-12-10
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