PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Schweiger, W.* ; Pasquet, J.C.* ; Nussbaumer, T. ; Kovalsky Paris, M.P.* ; Wiesenberger, G.* ; Macadré, C.* ; Ametz, C.* ; Berthiller, F.* ; Lemmens, M.* ; Saindrenan, P.* ; Mewes, H.-W. ; Mayer, K.F.X. ; Dufresne, M.* ; Adam, G.*

Functional characterization of two clusters of Brachypodium distachyon UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes.

Mol. Plant Microbe Interact. 26, 781-792 (2013)
Verlagsversion Volltext DOI PMC
Open Access Gold
Plant small molecule UDP-glycosyltransferases (UGT) glycosylate a vast number of endogenous substances but also act in detoxification of metabolites produced by plant pathogenic microorganisms. The ability to inactivate the Fusarium graminearum mycotoxin deoxynivalenol (DON) into DON-3-O-glucoside is crucial for resistance of cereals. We analyzed the UGT gene family of the monocot model species Brachypodium distachyon and functionally characterized two gene clusters containing putative orthologs of previously identified DON-detoxification genes from Arabidopsis thaliana and barley. Analysis of transcription showed that UGTs encoded in both clusters are highly inducible by DON and expressed at much higher levels upon infection with a wild-type DON-producing F. graminearum strain compared to infection with a mutant deficient in DON production. Expression of these genes in a toxin sensitive strain of Saccharomyces cerevisiae revealed that only two B. distachyon UGTs encoded by members of a cluster of six genes homologous to the DON-inactivating barley HvUGT13248 were able to convert DON into DON-3-O-glucoside. Also, a single copy gene from Sorghum bicolor orthologous to this cluster and one of three putative orthologs of rice exhibit this ability. Seemingly, the UGT genes undergo rapid evolution and changes in copy number, making it difficult to identify orthologs with conserved substrate specificity.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
4.307
1.235
49
60
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Head Blight Resistance ; Fusarium Mycotoxin Deoxynivalenol ; Transcriptome Analysis ; Arabidopsis-thaliana ; Plant Glycosyltransferases ; Heterologous Expression ; Cause Disease ; Wheat Spikes ; Graminearum ; Glucosyltransferase
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 0894-0282
e-ISSN 1943-7706
Zeitschrift Molecular Plant-Microbe Interactions
Quellenangaben Band: 26, Heft: 7, Seiten: 781-792 Artikelnummer: , Supplement: ,
Verlag American Phytopathological Society
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Bioinformatics and Systems Biology (IBIS)
POF Topic(s) 20402 - Sustainable Plant Production
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503790-001
PubMed ID 23550529
DOI 10.1094/MPMI-08-12-0205-R
WOS ID WOS:000320411800007
Scopus ID 84878656400
Erfassungsdatum 2013-04-12
[➜Korrektur melden]