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Venit, T.* ; Dzijak, R.* ; Kalendova, A.* ; Kahle-Stephan, M.* ; Rohozkova, J.* ; Schmidt, V.* ; Rülicke, T.* ; Rathkolb, B. ; Hans, W. ; Bohla, A. ; Eickelberg, O. ; Stöger, T. ; Wolf, E.* ; Yildirim, A.Ö. ; Gailus-Durner, V. ; Fuchs, H. ; Hrabě de Angelis, M. ; Hozak, P.*

Mouse nuclear myosin 1 knock-out shows interchangeability and redundancy of myosin isoforms in the cell nucleus.

PLoS ONE 8:e61406 (2013)
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Background: Nuclear myosin I (NM1) is a nuclear isoform of the well-known "cytoplasmic" Myosin 1c protein (Myo1c). Located on the 11th chromosome in mice, NM1 results from an alternative start of transcription of the Myo1c gene adding an extra 16 amino acids at the N-terminus. Previous studies revealed its roles in RNA Polymerase I and RNA Polymerase II transcription, chromatin remodeling, and chromosomal movements. Its nuclear localization signal is localized in the middle of the molecule and therefore directs both Myosin 1c isoforms to the nucleus. Methodology/Principal Findings: In order to trace specific functions of the NM1 isoform, we generated mice lacking the NM1 start codon without affecting the cytoplasmic Myo1c protein. Mutant mice were analyzed in a comprehensive phenotypic screen in cooperation with the German Mouse Clinic. Strikingly, no obvious phenotype related to previously described functions has been observed. However, we found minor changes in bone mineral density and the number and size of red blood cells in knock-out mice, which are most probably not related to previously described functions of NM1 in the nucleus. In Myo1c/NM1 depleted U2OS cells, the level of Pol I transcription was restored by overexpression of shRNA-resistant mouse Myo1c. Moreover, we found Myo1c interacting with Pol II. The ratio between Myo1c and NM1 proteins were similar in the nucleus and deletion of NM1 did not cause any compensatory overexpression of Myo1c protein. Conclusion/Significance: We observed that Myo1c can replace NM1 in its nuclear functions. Amount of both proteins is nearly equal and NM1 knock-out does not cause any compensatory overexpression of Myo1c. We therefore suggest that both isoforms can substitute each other in nuclear processes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Actin ; Transcription ; Myo1c ; Identification ; Membrane ; Insulin ; Mutations ; Transport ; Network ; Complex
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 1932-6203
Zeitschrift PLoS ONE
Quellenangaben Band: 8, Heft: 4, Seiten: , Artikelnummer: e61406 Supplement: ,
Verlag Public Library of Science (PLoS)
Verlagsort Lawrence, Kan.
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Experimental Genetics (IEG)
Institute of Lung Health and Immunity (LHI)
POF Topic(s) 30201 - Metabolic Health
30202 - Environmental Health
Forschungsfeld(er) Genetics and Epidemiology
Lung Research
PSP-Element(e) G-500600-003
G-500600-001
G-501600-001
G-505000-001
G-505000-007
G-505000-006
PubMed ID 23593477
DOI 10.1371/journal.pone.0061406
WOS ID WOS:000317383200056
Scopus ID 84876126387
Erfassungsdatum 2013-04-22
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