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Wolf, A. ; Mantri, M.* ; Heim, A.* ; Müller, U.* ; Fichter, E.* ; Mackeen, M.M.* ; Schermelleh, L.* ; Dadie, G.* ; Leonhardt, H.* ; Vénien-Bryan, C.* ; Kessler, B.M.* ; Schofield, C.J.* ; Böttger, A.*

The poly-serine domain of the lysyl-5 hydroxylase Jmjd6 mediates subnuclear localization.

Biochem. J. 453, 357-370 (2013)
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Jmjd6 is an Fe(II) and 2-oxoglutarate (2OG) dependent oxygenase that catalyses hydroxylation of lysine residues in proteins involved in pre-mRNA splicing. Jmjd6 plays an essential role in vertebrate embryonic development and has been shown to modulate alternative splicing in response to hypoxic stress. Here we show that an alternatively spliced version of Jmjd6 lacking the polyS domain localises to the nucleolus, predominantly in the fibrillar centre. PolyS domain deleted Jmjd6 also interacts with nucleolar proteins. Furthermore, co-immunoprecipitation experiments and fluorescent 2-hybrid (F2H) assays demonstrate that Jmjd6 homo-oligomerisation occurs in cells. In correlation with the observed variations in the subnuclear distribution of Jmjd6 the structure of Jmjd6 oligomers in vitro changes in the absence of the polyS domain, possibly reflecting the role of the polyS domain in nuclear/nucleolar shuttling of Jmjd6.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Fe(ii)- And 2-oxoglutarate-dependent Oxygenase ; Jmjc ; Lysine Hydroxylation ; Nucleolus ; Polyserine Domain ; Pre-mrna Splicing; Hypoxia-inducible Factor ; Living Cells ; Phosphatidylserine Receptor ; Protein Interactions ; Mass-spectrometry ; Rna ; Visualization ; Nucleolus ; 2-oxoglutarate ; Identification
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 0264-6021
e-ISSN 1470-8728
Zeitschrift Biochemical Journal / Reviews
Quellenangaben Band: 453, Heft: 3, Seiten: 357-370 Artikelnummer: , Supplement: ,
Verlag Portland Press
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Toxicology and Pharmacology (TOX)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-552500-001
PubMed ID 23688307
DOI 10.1042/BJ20130529
WOS ID WOS:000322358700005
Scopus ID 84880063803
Erfassungsdatum 2013-07-15
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