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Ellinghaus, D.* ; Zhang, H.* ; Zeissig, S.* ; Lipinski, S.* ; Till, A.* ; Jiang, T.* ; Stade, B.* ; Bromberg, Y.* ; Ellinghaus, E.* ; Keller, A.* ; Rivas, M.A.* ; Skieceviciene, J.* ; Doncheva, N.T.* ; Liu, X.* ; Liu, Q.* ; Jiang, F.* ; Forster, M.* ; Mayr, G.* ; Albrecht, M.* ; Häsler, R.* ; Boehm, B.O.* ; Goodall, J.* ; Berzuini, C.R.* ; Lee, J.* ; Andersen, V.* ; Vogel, U.* ; Kupcinskas, L.* ; Kayser, M.* ; Krawczak, M.* ; Nikolaus, S.* ; Weersma, R.K.* ; Ponsioen, C.Y.* ; Sans, M.* ; Wijmenga, C.* ; Strachan, D.P.* ; McArdle, W.L.* ; Vermeire, S.* ; Rutgeerts, P.* ; Sanderson, J.D.* ; Mathew, C.G.* ; Vatn, M.H.* ; Wang, J.* ; Nöthen, M.M.* ; Duerr, R.H.* ; Büning, C.* ; Brand, S.* ; Glas, J.* ; Winkelmann, J. ; Illig, T. ; Latiano, A.* ; Annese, V.* ; Halfvarson, J.* ; D'Amato, M.* ; Daly, M.J.* ; Nothnagel, M.* ; Karlsen, T.H.* ; Subramani, S.* ; Rosenstiel, P.* ; Schreiber, S.* ; Parkes, M.* ; Franke, A.*

Association between variants of PRDM1 and NDP52 and Crohn's disease, based on exome sequencing and functional studies.

Gastroenterology 145, 339-347 (2013)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
BACKGROUND & AIMS: Genome-wide association studies (GWAS) have identified 140 Crohn's disease (CD) susceptibility loci. For most loci, the variants that cause disease are not known and the genes affected by these variants have not been identified. We aimed to identify variants that cause CD through detailed sequencing, genetic association, expression, and functional studies. METHODS: We sequenced whole exomes of 42 unrelated subjects with CD and 5 healthy subjects (controls) and then filtered single nucleotide variants by incorporating association results from meta-analyses of CD GWAS and in silico mutation effect prediction algorithms. We then genotyped 9348 subjects with CD, 2868 subjects with ulcerative colitis, and 14,567 control subjects and associated variants analyzed in functional studies using materials from subjects and controls and in vitro model systems. RESULTS: We identified rare missense mutations in PR domain-containing 1 (PRDM1) and associated these with CD. These mutations increased proliferation of T cells and secretion of cytokines on activation and increased expression of the adhesion molecule L-selectin. A common CD risk allele, identified in GWAS, correlated with reduced expression of PRDM1 in ileal biopsy specimens and peripheral blood mononuclear cells (combined P = 1.6 × 10(-8)). We identified an association between CD and a common missense variant, Val248Ala, in nuclear domain 10 protein 52 (NDP52) (P = 4.83 × 10(-9)). We found that this variant impairs the regulatory functions of NDP52 to inhibit nuclear factor κB activation of genes that regulate inflammation and affect the stability of proteins in Toll-like receptor pathways. CONCLUSIONS: We have extended the results of GWAS and provide evidence that variants in PRDM1 and NDP52 determine susceptibility to CD. PRDM1 maps adjacent to a CD interval identified in GWAS and encodes a transcription factor expressed by T and B cells. NDP52 is an adaptor protein that functions in selective autophagy of intracellular bacteria and signaling molecules, supporting the role of autophagy in the pathogenesis of CD.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter CD; Complex Disease; Crohn’s disease; GWAS; IBD; IFN; Inflammatory Bowel Disease; NF-κB; PBL; PBMC; SNP; SNV; TLR; Toll-like receptor; UC; WT; Whole-Exome Sequencing; eQTL; expression quantitative trait locus; genome-wide association studies; inflammatory bowel disease; interferon; nuclear factor κB; peripheral blood lymphocyte; peripheral blood mononuclear cell; qRT-PCR; quantitative reverse-transcription polymerase chain reaction; single nucleotide polymorphism; single nucleotide variant; ulcerative colit; Genome-wide Association ; Transcriptional Repressor Blimp-1 ; Inflammatory-bowel-disease ; T-cell Homeostasis ; Susceptibility Loci ; Lymphocyte Migration ; Ulcerative-colitis ; Autophagy Receptor ; L-selectin ; Expression
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 0016-5085
e-ISSN 1528-0012
Zeitschrift Gastroenterology
Quellenangaben Band: 145, Heft: 2, Seiten: 339-347 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Human Genetics (IHG)
Research Unit Molecular Epidemiology (AME)
POF Topic(s) 30501 - Systemic Analysis of Genetic and Environmental Factors that Impact Health
Forschungsfeld(er) Genetics and Epidemiology
PSP-Element(e) G-500700-001
G-504200-001
PubMed ID 23624108
DOI 10.1053/j.gastro.2013.04.040,S0016-5085(13)00620-3
WOS ID WOS:000322630600023
Erfassungsdatum 2013-08-02
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