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Zischka, H. ; Larochette, N.* ; Hoffmann, F.* ; Hamöller, D. ; Jägemann, N. ; Lichtmannegger, J. ; Jennen, L. ; Müller-Höcker, J.* ; Roggel, F.* ; Göttlicher, M. ; Vollmar, A.M.* ; Kroemer, G.*

Electrophoretic analysis of the mitochondrial outer membrane rupture induced by permeability transition.

Anal. Chem. 80, 5051-5058 (2008)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A pathological increase of the permeability of the mitochondrial membranes may culminate in the irreversible rupture of the mitochondrial outer membrane. Such a permeability transition is lethal because it results in the release of death-inducing molecules from mitochondria and/or metabolic failure. Current methods to assess this outer membrane damage are mostly indirect or scarcely representative of the overall mitochondrial population. Here we present an analytical and preparative approach using free flow electrophoresis to directly distinguish rat liver mitochondria that have undergone the permeability transition from unaffected organelles or from organelles that are damaged to a minor degree. Mitochondrial populations, which considerably differ in outer membrane integrity or cytochrome c content, were separated by this means. We further show that the relative abundance of each population depends on the dose of the permeability transition inducer and the duration of the treatment time. Finally, we have employed this approach to investigate the impairment of mitochondria that were isolated from livers subjected to ischemia/reperfusion damage.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 2008
HGF-Berichtsjahr 2008
ISSN (print) / ISBN 0003-2700
e-ISSN 1520-6882
Zeitschrift Analytical Chemistry
Quellenangaben Band: 80, Heft: 13, Seiten: 5051-5058 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pathology (PATH)
Institute of Molecular Toxicology and Pharmacology (TOX)
Research Unit Analytical Pathology (AAP)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30203 - Molecular Targets and Therapies
30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-500300-001
G-505200-001
G-500390-001
DOI 10.1021/ac800173r
Scopus ID 46849114908
Erfassungsdatum 2008-08-29
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