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A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies.
Anal. Biochem. 443, 66-74 (2013)
Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
2.582
0.942
45
44
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Mitochondria ; Cell Culture ; Biopsies ; Balch Homogenizer; Stimulated Glut4 Translocation ; Permeability Transition ; Rat Liver ; Electrophoresis ; Quantitation ; Membrane ; Proteins ; Death
Sprache
englisch
Veröffentlichungsjahr
2013
HGF-Berichtsjahr
2013
ISSN (print) / ISBN
0003-2697
e-ISSN
1096-0309
Zeitschrift
Analytical Biochemistry
Quellenangaben
Band: 443,
Heft: 1,
Seiten: 66-74
Verlag
Elsevier
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Molecular Toxicology and Pharmacology (TOX)
Molekulare Endokrinologie und Metabolismus (MEM)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
Molekulare Endokrinologie und Metabolismus (MEM)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
POF Topic(s)
30203 - Molecular Targets and Therapies
30201 - Metabolic Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30205 - Bioengineering and Digital Health
30201 - Metabolic Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30205 - Bioengineering and Digital Health
Forschungsfeld(er)
Enabling and Novel Technologies
Genetics and Epidemiology
Genetics and Epidemiology
PSP-Element(e)
G-505200-003
G-505600-001
G-500300-001
G-500390-001
G-505600-001
G-500300-001
G-500390-001
PubMed ID
23969012
WOS ID
WOS:000326213900011
Scopus ID
84884599409
Erfassungsdatum
2013-09-05