PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Mascher, M.* ; Richmond, T.A.* ; Gerhardt, D.J.* ; Himmelbach, A.* ; Clissold, L.* ; Sampath, D.* ; Ayling, S.* ; Steuernagel, B.* ; Pfeifer, M. ; D'Ascenzo, M.* ; Akhunov, E.D.* ; Hedley, P.E.* ; Gonzales, A.M.* ; Morrell, P.L.* ; Kilian, B.* ; Blattner, F.R.* ; Scholz, U.* ; Mayer, K.F.X. ; Flavell, A.J.* ; Muehlbauer, G.J.* ; Waugh, R.* ; Jeddeloh, J.A.* ; Stein, N.*

Barley whole exome capture: A tool for genomic research in the genus Hordeum and beyond.

Plant J. 76, 494-505 (2013)
Verlagsversion Volltext DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Advanced resources for genome-assisted research in barley (Hordeum vulgare) including a whole-genome shotgun assembly and an integrated physical map have recently become available. These have made possible studies that aim to assess genetic diversity or to isolate single genes by whole-genome resequencing and in silico variant detection. However such an approach remains expensive given the 5 Gb size of the barley genome. Targeted sequencing of the mRNA-coding exome reduces barley genomic complexity more than 50-fold, thus dramatically reducing this heavy sequencing and analysis load. We have developed and employed an in-solution hybridization-based sequence capture platform to selectively enrich for a 61.6 megabase coding sequence target that includes predicted genes from the genome assembly of the cultivar Morex as well as publicly available full-length cDNAs and de novo assembled RNA-Seq consensus sequence contigs. The platform provides a highly specific capture with substantial and reproducible enrichment of targeted exons, both for cultivated barley and related species. We show that this exome capture platform provides a clear path towards a broader and deeper understanding of the natural variation residing in the mRNA-coding part of the barley genome and will thus constitute a valuable resource for applications such as mapping-by-sequencing and genetic diversity analyzes.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
6.582
1.650
168
181
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Hordeum bulbosum; Hordeum pubiflorum; Hordeum vulgare; Triticeae; barley; genetic diversity; genomics; targeted resequencing; Mutation Discovery ; Natural Variation ; Wild Barley ; Maize ; Diversity ; Poaceae ; Domestication ; Nucleotide ; Map
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 0960-7412
e-ISSN 1365-313X
Zeitschrift Plant Journal, The
Quellenangaben Band: 76, Heft: 3, Seiten: 494-505 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Bioinformatics and Systems Biology (IBIS)
POF Topic(s) 20402 - Sustainable Plant Production
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503790-001
PubMed ID 23889683
DOI 10.1111/tpj.12294
WOS ID WOS:000326246700012
Scopus ID 84885672827
Erfassungsdatum 2013-09-06
[➜Korrektur melden]