PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Romm, H.* ; Barnard, S.* ; Boulay-Greene, H.* ; de Amicis, A.* ; de Sanctis, S.* ; Franco, M.* ; Herodin, F.* ; Jones, A.* ; Kulka, U.* ; Lista, F.* ; Martigne, P.* ; Moquet, J.* ; Oestreicher, U.* ; Rothkamm, K.* ; Thierens, H.* ; Valente, M.* ; Vandersickel, V.* ; Vral, A.* ; Braselmann, H. ; Meineke, V.* ; Abend, M.* ; Beinke, C.*

Laboratory intercomparison of the cytokinesis-block micronucleus assay.

Radiat. Res. 180, 120-128 (2013)
Verlagsversion DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The focus of the study is an intercomparison of laboratories' dose-assessment performances using the cytokinesis-block micronucleus (CBMN) assay as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-5 Gy) as well as blind samples (0.1-6.4 Gy) were sent to the participants. The CBMN assay was performed according to protocols individually established and varying among participating laboratories. The time taken to report dose estimates was documented for each laboratory. Additional information concerning laboratory organization/characteristics as well as assay performance was collected. The mean absolute difference (MAD) was calculated and radiation doses were merged into four triage categories reflecting clinical aspects to calculate accuracy, sensitivity and specificity. The earliest report time was 4 days after sample arrival. The CBMN dose estimates were reported with high accuracy (MAD values of 0.20-0.50 Gy at doses below 6.4 Gy for both manual and automated scoring procedures), but showed a limitation of the assay at the dose point of 6.4 Gy, which resulted in a clear dose underestimation in all cases. The MAD values (without 6.4 Gy) differed significantly (P = 0.03) between manual (0.25 Gy, SEM = 0.06, n = 4) or automated scoring procedures (0.37 Gy, SEM = 0.08, n = 5), but lowest MAD were equal (0.2 Gy) for both scoring procedures. Likewise, both scoring procedures led to the same allocation of dose estimates to triage categories of clinical significance (about 83% accuracy and up to 100% specificity).
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
2.698
1.326
30
35
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Peripheral-blood Lymphocytes; Radiation Biodosimetry; Biological Dosimetry; Ionizing-radiation; Image-analysis; Sensitivity; Accidents; Exposure; Network; Emergencies
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 0033-7587
e-ISSN 1938-5404
Zeitschrift Radiation Research
Quellenangaben Band: 180, Heft: 2, Seiten: 120-128 Artikelnummer: , Supplement: ,
Verlag Radiation Research Society
Verlagsort Lawrence
Begutachtungsstatus Peer reviewed
Institut(e) Translational Metabolic Oncology (IDC-TMO)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Radiation Sciences
PSP-Element(e) G-501000-001
PubMed ID 23862731
DOI 10.1667/RR3234.1
WOS ID WOS:000330990700002
Scopus ID 84882283493
Erfassungsdatum 2013-09-23
[➜Korrektur melden]