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Uhlmann, T. ; Boeing, S. ; Lehmbacher, M. ; Meisterernst, M.

The VP16 activation domain establishes an active mediator lacking CDK8 in vivo.

J. Biol. Chem. 282, 2163-2173 (2007)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
VP16 has been widely used to unravel the mechanisms underlying gene transcription. Much of the previous work has been conducted in reconstituted in vitro systems. Here we study the formation of transcription complexes at stable reporters under the control of an inducible Tet-VP16 activator in living cells. In this simplified model for gene activation VP16 recruits the general factors and the cofactors Mediator, GCN5, CBP, and PC4, within minutes to the promoter region. Activation is accompanied by only minor changes in histone acetylation and H3K4 methylation but induces a marked promoter-specific increase in H3K79 methylation. Mediated through contacts with VP16 several subunits of the cleavage and polyadenylation factor (CPSF/CstF) are concentrated at the promoter region. We provide in vitro and in vivo evidence that VP16 activates transcription through a specific MED25-associated Mediator, which is deficient in CDK8.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 2007
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0021-9258
e-ISSN 1083-351X
Zeitschrift Journal of Biological Chemistry, The
Quellenangaben Band: 282, Heft: , Seiten: 2163-2173 Artikelnummer: , Supplement: ,
Verlag American Society for Biochemistry and Molecular Biology
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Immunology (IMI)
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) FE 71781
PubMed ID 17135252
DOI 10.1074/jbc.M608451200
WOS ID 000243593200007
Erfassungsdatum 2007-06-06
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