PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Börner, K.* ; Hermle, J.* ; Sommer, C.* ; Brown, N.P.* ; Knapp, B.* ; Glass, B.* ; Kunkel, J.* ; Torralba, G.* ; Reymann, J.* ; Beil, N.* ; Beneke, J.* ; Pepperkok, R.* ; Schneider, R.* ; Ludwig, T.* ; Hausmann, M.* ; Hamprecht, F.* ; Erfle, H.* ; Kaderali, L.* ; Kräusslich, H.G.* ; Lehmann, M.J.*

From experimental setup to bioinformatics: An RNAi screening platform to identify host factors involved in HIV-1 replication.

Biotechnol. J. 5, 39-49 (2010)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
RNA interference (RNAi) has emerged as a powerful technique for studying loss-of-function phenotypes by specific down-regulation of gene expression, allowing the investigation of virus-host interactions by large-scale high-throughput RNAi screens. Here we present a robust and sensitive small interfering RNA screening platform consisting of an experimental setup, single-cell image and statistical analysis as well as bioinformatics. The workflow has been established to elucidate host gene functions exploited by viruses, monitoring both suppression and enhancement of viral replication simultaneously by fluorescence microscopy. The platform comprises a two-stage procedure in which potential host factors are first identified in a primary screen and afterwards re-tested in a validation screen to confirm true positive hits. Subsequent bioinformatics allows the identification of cellular genes participating in metabolic pathways and cellular networks utilised by viruses for efficient infection. Our workflow has been used to investigate host factor usage by the human immunodeficiency virus-1 (HIV-1), but can also be adapted to other viruses. Importantly, we expect that the description of the platform will guide further screening approaches for virus-host interactions. The ViroQuant-CellNetworks RNAi Screening core facility is an integral part of the recently founded BioQuant centre for systems biology at the University of Heidelberg and will provide service to external users in the near future.
Impact Factor
Scopus SNIP
Scopus
Cited By
Altmetric
0.000
0.490
36
Tags
Icb_extern
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 2010
HGF-Berichtsjahr 0
ISSN (print) / ISBN 1860-6768
Zeitschrift Biotechnology Journal
Quellenangaben Band: 5, Heft: 1, Seiten: 39-49 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Computational Biology (ICB)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503800-001
PubMed ID 20013946
DOI 10.1002/biot.200900226
Erfassungsdatum 2010-12-31
[➜Korrektur melden]