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Peng, X.* ; Mandal, P.K. ; Kaminskyy, V.O.* ; Lindqvist, A.* ; Conrad, M. ; Arner, E.S.J.*

Sec-containing TrxR1 is essential for self-sufficiency of cells by control of glucose-derived H2O2.

Cell Death Dis. 5:e1235 (2014)
Verlagsversion Volltext DOI PMC
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It is commonly recognized that diabetic complications involve increased oxidative stress directly triggered by hyperglycemia. The most important cellular protective systems against such oxidative stress have yet remained unclear. Here we show that the selenoprotein thioredoxin reductase 1 (TrxR1), encoded by the Txnrd1 gene, is an essential enzyme for such protection. Individually grown Txnrd1 knockout (Txnrd1(-/-)) mouse embryonic fibroblasts (MEFs) underwent massive cell death directly linked to glucose-induced H2O2 production. This death and excessive H2O2 levels could be reverted by reconstituted expression of selenocysteine (Sec)-containing TrxR1, but not by expression of Sec-devoid variants of the enzyme. Our results show that Sec-containing TrxR1 is absolutely required for self-sufficient growth of MEFs under high-glucose conditions, owing to an essential importance of this enzyme for elimination of glucose-derived H2O2. To our knowledge, this is the first time a strict Sec-dependent function of TrxR1 has been identified as being essential for mammalian cells.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Glucose ; Mouse Embryonic Fibroblasts (mefs) ; Reactive Oxygen Species (ros) ; Selenocysteine ; Thioredoxin Reductase 1 (trxr1); Thioredoxin Reductase 1; Reactive Oxygen; Mammalian Thioredoxin; Hydrogen-peroxide; Diabetic Complications; Dna-replication; Stem-cells; Glutathione; Death; Apoptosis
Sprache englisch
Veröffentlichungsjahr 2014
HGF-Berichtsjahr 2014
ISSN (print) / ISBN 2041-4889
e-ISSN 2041-4889
Zeitschrift Cell Death & Disease
Quellenangaben Band: 5, Heft: , Seiten: , Artikelnummer: e1235 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
Institute of Developmental Genetics (IDG)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30204 - Cell Programming and Repair
Forschungsfeld(er) Immune Response and Infection
Genetics and Epidemiology
PSP-Element(e) G-501490-001
G-500500-001
G-500500-004
PubMed ID 24853413
DOI 10.1038/cddis.2014.209
WOS ID WOS:000337229300035
Scopus ID 84901049603
Erfassungsdatum 2014-05-25
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