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Chen, H.* ; Kevan-Jah, S.* ; Suentzenich, K.O. ; Grässer, F.A.* ; Mueller- Lantzsch, N.G.*

Expression of the Epstein-Barr virus latent membrane protein (LMP) in insect cells and detection of antibodies in human sera against this protein.

Virology 190, 106-115 (1992)

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Recombinant baculoviruses containing the complete LMP and truncated LIMP genes were generated and high levels of the LMP proteins were expressed in Spadoptera Frugiperda insect cells. A specific rabbit antiserum directed against the N-terminal part of LMP was obtained by immunizing the rabbits with Escherichia coli-expressed trpE-N-terminal part of LMP fusion protein. A total of 127 human sera were studied for their immune response to the recombinant full-length LMP. In immunofluorescence analysis, all sera tested showed no detectable reaction with the recombinant full-length LMP. In immunoprecipitation-immunoblotting analysis, however, sera from patients with nasopharyngeal carcinoma ( 5 22), patients with Hodgkin's disease ( 16 27), patients with other diseases exhibiting high EA-IgG titers ( 3 52), and VCA-IgG-positive healthy individuals ( 2 26) were shown to contain antibodies against this recombinant LMP. The expressed LMP proteins provided a sufficient and economic source of the proteins for further serological and biological studies.

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