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Time-resolved microfluorescence for measuring coenzyme F420 in Methanobacterium thermoautotrophicum.
FEMS Microbiol. Lett. 22, 205-208 (1984)
The time course of photobleaching and the nanosecond fluorescence decay have been measured from microscopic samples of methanogenic bacteria, to our knowledge the first application of these methods in this field. Decay times of about 1 ns and 3 ns were obtained for the specific coenzymes F420 and 7-methylpterin, respectively. In contrast to methylpterin and other fluorescent compounds the intensity of F420 fluorescence was reduced selectively due to photobleaching. This effect, as well as the different decay time constants could be used to discriminate F420 from other fluorescent components. In addition, active and inactive bacterial cells could be differentiated following the course of photobleaching.
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Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
7-methylpterin ; Autofluorescence ; Fluorescence Decay ; Methanogenic Bacteria ; Photobleaching
Sprache
englisch
Veröffentlichungsjahr
1984
HGF-Berichtsjahr
0
ISSN (print) / ISBN
0378-1097
e-ISSN
1574-6968
Zeitschrift
FEMS Microbiology Letters
Quellenangaben
Band: 22,
Heft: 3,
Seiten: 205-208
Verlag
Wiley
Begutachtungsstatus
Peer reviewed
Institut(e)
Institut für angewandte Optik
Scopus ID
0021271116
Erfassungsdatum
1984-12-31