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Divakaruni, A.S.* ; Paradyse, A.* ; Ferrick, D.A.* ; Murphy, A.N.* ; Jastroch, M.

Analysis and interpretation of microplate-based oxygen consumption and pH data.

Methods Enzymol. 547, 309-354 (2014)
DOI PMC
Breakthrough technologies to measure cellular oxygen consumption and proton efflux are reigniting the study of cellular energetics by increasing the scope and pace with which discoveries are made. As we learn the variation in metabolism between cell types is large, it is helpful to continually provide additional perspectives and update our roadmap for data interpretation. In that spirit, this chapter provides the following for those conducting microplate-based oxygen consumption experiments: (i) a description of the standard parameters for measuring respiration in intact cells, (ii) a framework for data analysis and normalization, and (iii) examples of measuring respiration in permeabilized cells to follow up results observed with intact cells. Additionally, rate-based measurements of extracellular pH are increasingly used as a qualitative indicator of glycolytic flux. As a resource to help interpret these measurements, this chapter also provides a detailed accounting of proton production during glucose oxidation in the context of plate-based assays.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Bioenergetics ; Ecar ; Glycolysis ; Lactate Efflux ; Mitochondria ; Ocr ; Permeabilized Cells ; Proton Leak ; Respiration ; Seahorse Xf Analyzer
Sprache englisch
Veröffentlichungsjahr 2014
HGF-Berichtsjahr 2014
ISSN (print) / ISBN 0076-6879
e-ISSN 0076-6879
Zeitschrift Methods in Enzymology
Quellenangaben Band: 547, Heft: , Seiten: 309-354 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Obesity (IDO)
POF Topic(s) 30201 - Metabolic Health
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502200-001
PubMed ID 25416364
DOI 10.1016/B978-0-12-801415-8.00016-3
Scopus ID 84938491158
Scopus ID 84925400550
Erfassungsdatum 2014-11-26
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