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Linnemann, J. ; Miura, H. ; Meixner, L.K. ; Irmler, M. ; Kloos, U. ; Hirschi, B. ; Bartsch, H.S.* ; Sass, S. ; Beckers, J. ; Theis, F.J. ; Gabka, C.* ; Sotlar, K.* ; Scheel, C.

Quantification of regenerative potential in primary human mammary epithelial cells.

Development 142, 3239-3251 (2015)
Verlagsversion DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
We present an organoid regeneration assay in which freshly isolated human mammary epithelial cells are cultured in adherent or floating collagen gels, corresponding to a rigid or compliant matrix environment. In both conditions, luminal progenitors form spheres, whereas basal cells generate branched ductal structures. In compliant but not rigid collagen gels, branching ducts form alveoli at their tips, express basal and luminal markers at correct positions, and display contractility, which is required for alveologenesis. Thereby, branched structures generated in compliant collagen gels resemble terminal ductal-lobular units (TDLUs), the functional units of the mammary gland. Using the membrane metallo-endopeptidase CD10 as a surface marker enriches for TDLU formation and reveals the presence of stromal cells within the CD49f(hi)/EpCAM(-) population. In summary, we describe a defined in vitro assay system to quantify cells with regenerative potential and systematically investigate their interaction with the physical environment at distinct steps of morphogenesis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Basal ; Branching Morphogenesis ; Cd10 ; Collagen Gel ; Luminal ; Mammary Stem Cell ; Organoid ; Primary Human Mammary Epithelial Cells ; Progenitor Cells ; Regenerative Potential ; Terminal Ductal-lobular Units
Sprache englisch
Veröffentlichungsjahr 2015
HGF-Berichtsjahr 2015
ISSN (print) / ISBN 0950-1991
e-ISSN 1477-9129
Zeitschrift Development / Company of Biologists
Quellenangaben Band: 142, Heft: 18, Seiten: 3239-3251 Artikelnummer: , Supplement: ,
Verlag Company of Biologists
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Stem Cell Research (ISF)
Institute of Experimental Genetics (IEG)
Institute of Computational Biology (ICB)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30201 - Metabolic Health
30205 - Bioengineering and Digital Health
Forschungsfeld(er) Stem Cell and Neuroscience
Genetics and Epidemiology
Enabling and Novel Technologies
PSP-Element(e) G-552300-001
G-500600-004
G-503800-001
PubMed ID 26071498
DOI 10.1242/dev.123554
WOS ID WOS:000361742600018
Scopus ID 84942155417
Erfassungsdatum 2015-06-15
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