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Kneipp, M. ; Estrada, H. ; Lauri, A. ; Turner, J.E. ; Ntziachristos, V. ; Westmeyer, G.G. ; Razansky, D.

Volumetric tracking of migratory melanophores during zebrafish development by optoacoustic microscopy.

Mech. Dev. 138, 300-304 (2015)
Verlagsversion DOI PMC
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Unveiling mechanisms driving specification, recruitment and regeneration of melanophores is key in understanding melanin-related disorders. This study reports on the applicability of a hybrid focus optoacoustic microscope (HFOAM) for volumetric tracking of migratory melanophores in developing zebrafish. The excellent contrast from highly-absorbing melanin provided by the method is shown to be ideal for label-free dynamic visualization of melanophores in their unperturbed environment. We established safe laser energy levels that enable high-contrast longitudinal tracking of the cells over an extended period of developmental time without causing cell toxicity or pigment bleaching. Owing to its hybrid optical and acoustic resolution, the new imaging technique can be seamlessly applied for noninvasive studies of both optically-transparent larval as well as adult stages of the zebrafish model organism, which is not possible using other optical microscopy methods.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Label-free ; Melanocytes ; Optoacoustic Microscopy ; Tracking ; Volumetric
Sprache englisch
Veröffentlichungsjahr 2015
HGF-Berichtsjahr 2015
ISSN (print) / ISBN 0925-4773
e-ISSN 1872-6356
Zeitschrift Mechanisms of Development
Quellenangaben Band: 138, Heft: , Seiten: 300-304 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Institute of Developmental Genetics (IDG)
POF Topic(s) 30205 - Bioengineering and Digital Health
30505 - New Technologies for Biomedical Discoveries
30204 - Cell Programming and Repair
Forschungsfeld(er) Enabling and Novel Technologies
Genetics and Epidemiology
PSP-Element(e) G-505500-001
G-505590-001
G-552000-001
G-500500-001
PubMed ID 26376465
DOI 10.1016/j.mod.2015.09.001
WOS ID WOS:000365991800008
Scopus ID 84949312828
Scopus ID 84945336038
Erfassungsdatum 2015-10-08
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