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Sharma, S. ; Aneja, M.K. ; Mayer,J.* ; Schloter, M. ; Munch, J.-C.

RNA fingerprinting of microbial community in the rhizosphere soil of grain legumes.

FEMS Microbiol. Lett. 240, 181-186 (2004)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Microbial structural and expression profiles of the rhizospheres of three legumes, faba beans, peas and white lupin, were compared by RNA-arbitrarily primed PCR technique. Two different primers, M 13 reverse and 10-mer primers, were used in the amplification and products resolved on non-denaturing polyacrylamide gel. With both DNA and RNA profiles Lupinus and Pisum rhizospheres were more similar to each other than to Vicia rhizosphere. The RAP-PCR products were also dot blotted and probed for bacterial peptidase transcripts. Plant-dependent rhizosphere effect was evident by the marked absence of transcripts for bacterial neutral metallopeptidase in Lupinus rhizosphere. The results of dot blot were further confirmed by RT-PCR for the expression of bacterial neutral metallopeptidase in the three rhizospheres
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter RNA extraction; RAP-PCR; rhizosphere; bacterial proteases; expression profiles
Sprache englisch
Veröffentlichungsjahr 2004
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0378-1097
e-ISSN 1574-6968
Zeitschrift FEMS Microbiology Letters
Quellenangaben Band: 240, Heft: , Seiten: 181-186 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Soil Ecology (IBOE)
PSP-Element(e) G-504490-001
DOI 10.1016/j.femsle.2004.06.026
Erfassungsdatum 2004-11-04
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