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In situ detection of histone variants and modifications in mouse brain using imaging mass spectrometry.
Proteomics 16, 437-447 (2016)
Histone posttranslational modifications and histone variants control the epigenetic regulation of gene expression and affect a wide variety of biological processes. A complex pattern of such modifications and variants defines the identity of cells within complex organ systems and can therefore be used to characterize cells at a molecular level. However, their detection and identification in situ has been limited so far due to lack of specificity, selectivity and availability of anti-histone antibodies. Here, we describe a novel MALDI imaging mass spectrometry (MALDI-IMS) based workflow, which enables us to detect and characterize histones by their intact mass and their correlation with cytological properties of the tissue using novel statistical and image analysis tools. The workflow allows us to characterize the in situ distribution of the major histone variants and their modification in the mouse brain. This new analysis tool is particularly useful for the investigation of expression patterns of the linker histone H1 variants for which suitable antibodies are so far not available.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
4.079
0.919
14
18
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Hierarchical Clustering Analysis ; Histone Modifications ; Maldi Imaging Mass Spectrometry ; Nuclear Density ; Principal Component Analysis; Epigenetic Mechanisms; Nucleosome Core; H1; Tissue; H4; Reveals; Perspectives; Acetylation; Antibodies; Signatures
Sprache
englisch
Veröffentlichungsjahr
2016
Prepublished im Jahr
2015
HGF-Berichtsjahr
2015
ISSN (print) / ISBN
1615-9853
e-ISSN
1615-9861
Zeitschrift
Proteomics
Quellenangaben
Band: 16,
Heft: 3,
Seiten: 437-447
Verlag
Wiley
Verlagsort
Hoboken
Begutachtungsstatus
Peer reviewed
Institut(e)
Research Unit Analytical Pathology (AAP)
Institute of Stem Cell Research (ISF)
Institute of Pathology (PATH)
Institute of Stem Cell Research (ISF)
Institute of Pathology (PATH)
POF Topic(s)
30205 - Bioengineering and Digital Health
30204 - Cell Programming and Repair
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30204 - Cell Programming and Repair
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er)
Enabling and Novel Technologies
Stem Cell and Neuroscience
Stem Cell and Neuroscience
PSP-Element(e)
G-500390-001
G-500800-001
G-500300-001
G-500800-001
G-500300-001
WOS ID
WOS:000370130000007
Scopus ID
84958050008
Scopus ID
84955584977
PubMed ID
26593131
Erfassungsdatum
2015-12-02