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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Mechanism of transcriptional activation by FIS: role of core promoter structure and DNA topology.
J. Mol. Biol. 331, 331-344 (2003)
The Escherichia coli DNA architectural protein FIS activates transcription from stable RNA promoters on entry into exponential growth and also reduces the level of negative supercoiling. Here we show that such a reduction decreases the activity of the tyrT promoter but that activation by FIS rescues tyrT transcription at non-optimal superhelical densities. Additionally we show that three different "up" mutations in the tyrT core promoter either abolish or reduce the dependence of tyrT transcription on both high negative superhelicity and FIS in vivo and infer that the specific sequence organisation of the core promoter couples the control of transcription initiation by negative superhelicity and FIS. In vitro all the mutations potentiate FIS-independent untwisting of the -10 region while at the wild-type promoter FIS facilitates this step. We propose that this untwisting is a crucial limiting step in the initiation of tyrT RNA synthesis. The tyrT core promoter structure is thus optimised to combine high transcriptional activity with acute sensitivity to at least three major independent regulatory inputs: negative superhelicity, FIS and ppGpp.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Sprache
englisch
Veröffentlichungsjahr
2003
HGF-Berichtsjahr
0
ISSN (print) / ISBN
0022-2836
e-ISSN
1089-8638
Zeitschrift
Journal of Molecular Biology
Quellenangaben
Band: 331,
Heft: 2,
Seiten: 331-344
Verlag
Elsevier
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Functional Epigenetics (IFE)
POF Topic(s)
30203 - Molecular Targets and Therapies
Forschungsfeld(er)
Helmholtz Diabetes Center
PSP-Element(e)
G-502800-001
PubMed ID
12888342
Erfassungsdatum
2003-12-31