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Schneider, R.* ; Travers, A.* ; Kutateladze, T.* ; Muskhelishvili, G.*

A DNA architectural protein couples cellular physiology and DNA topology in Escherichia coli.

Mol. Microbiol. 34, 953-964 (1999)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
In Escherichia coli, the transcriptional activity of many promoters is strongly dependent on the negative superhelical density of chromosomal DNA. This, in turn, varies with the growth phase, and is correlated with the overall activity of DNA gyrase, the major topoisomerase involved in the elevation of negative superhelicity. The DNA architectural protein FIS is a regulator of the metabolic reorganization of the cell during early exponential growth phase. We have previously shown that FIS modulates the superhelical density of plasmid DNA in vivo, and on binding reshapes the supercoiled DNA in vitro. Here, we show that, in addition, FIS represses the gyrA and gyrB promoters and reduces DNA gyrase activity. Our results indicate that FIS determines DNA topology both by regulation of topoisomerase activity and, as previously inferred, by directly reshaping DNA. We propose that FIS is involved in coupling cellular physiology to the topology of the bacterial chromosome.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 1999
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0950-382x
e-ISSN 1365-2958
Zeitschrift Molecular Microbiology
Quellenangaben Band: 34, Heft: 5, Seiten: 953-964 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502800-001
PubMed ID 10594821
DOI 10.1046/j.1365-2958.1999.01656.x
Erfassungsdatum 1999-12-31
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