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Razansky, D. ; Distel, M. ; Vinegoni, C.* ; Ma, R.* ; Perrimon, N.* ; Köster, R.W. ; Ntziachristos, V.

Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo.

Nat. Photonics 3, 412-417 (2009)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Fluorescent proteins have become essential reporter molecules for studying life at the cellular and sub-cellular level, re-defining the ways in which we investigate biology. However, because of intense light scattering, most organisms and tissues remain inaccessible to current fluorescence microscopy techniques at depths beyond several hundred micrometres. We describe a multispectral opto-acoustic tomography technique capable of high-resolution visualization of fluorescent proteins deep within highly light-scattering living organisms. The method uses multiwavelength illumination over multiple projections combined with selective-plane opto-acoustic detection for artifact-free data collection. Accurate image reconstruction is enabled by making use of wavelength-dependent light propagation models in tissue. By performing whole-body imaging of two biologically important and optically diffuse model organisms, Drosophila melanogaster pupae and adult zebrafish, we demonstrate the facility to resolve tissue-specific expression of eGFP and mCherrry fluorescent proteins for precise morphological and functional observations in vivo.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter high-resolution; microscopy; brain
Sprache englisch
Veröffentlichungsjahr 2009
HGF-Berichtsjahr 2009
ISSN (print) / ISBN 1749-4885
e-ISSN 1749-4893
Zeitschrift Nature Photonics
Quellenangaben Band: 3, Heft: 7, Seiten: 412-417 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Institute of Developmental Genetics (IDG)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-505500-001
G-550200-001
PubMed ID ---
DOI 10.1038/NPHOTON.2009.98
WOS ID WOS:000268067700018
Scopus ID 68049118554
Erfassungsdatum 2009-12-31
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