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Gressel, S.* ; Schwalb, B.* ; Decker, T.-M. ; Qin, W.* ; Leonhardt, H.* ; Eick, D. ; Cramer, P.*

CDK9-dependent RNA polymerase II pausing controls transcription initiation.

eLife 6:e29736 (2017)
Verlagsversion Postprint Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Gene transcription can be activated by decreasing the duration of RNA polymerase II pausing in the promoter-proximal region, but how this is achieved remains unclear. Here we use a 'multi-omics' approach to demonstrate that the duration of polymerase pausing generally limits the productive frequency of transcription initiation in human cells ('pause-initiation limit'). We further engineer a human cell line to allow for specific and rapid inhibition of the P-TEFb kinase CDK9, which is implicated in polymerase pause release. CDK9 activity decreases the pause duration but also increases the productive initiation frequency. This shows that CDK9 stimulates release of paused polymerase and activates transcription by increasing the number of transcribing polymerases and thus the amount of mRNA synthesized per time. CDK9 activity is also associated with long-range chromatin interactions, suggesting that enhancers can influence the pause-initiation limit to regulate transcription.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Computational Biology ; Human ; Systems Biology; Genome-wide; Elongation Complex; In-vivo; Pol Ii; Drosophila; Maps; Crispr-cas9; Inhibitors; Resolution; Promoters
Sprache englisch
Veröffentlichungsjahr 2017
HGF-Berichtsjahr 2017
ISSN (print) / ISBN 2050-084X
e-ISSN 2050-084X
Zeitschrift eLife
Quellenangaben Band: 6, Heft: , Seiten: , Artikelnummer: e29736 Supplement: ,
Verlag eLife Sciences Publications
Verlagsort Cambridge
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502890-001
DOI 10.7554/eLife.29736
WOS ID WOS:000414407800001
Scopus ID 85036512014
Scopus ID 84869095110
PubMed ID 28994650
Erfassungsdatum 2017-10-17
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