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Philipp, J. ; Azimzadeh, O. ; Subramanian, V. ; Merl-Pham, J. ; Lowe, D.* ; Hladik, D. ; Erbeldinger, N.* ; Ktitareva, S.* ; Fournier, C.* ; Atkinson, M.J. ; Raj, K.* ; Tapio, S.

Radiation-induced endothelial inflammation is transferred via the secretome to recipient cells in a STAT-mediated process.

J. Proteome Res. 16, 3903-3916 (2017)

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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

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Radiation is the most common treatment of cancer. Minimizing the normal tissue injury, especially the damage to vascular endothelium, remains a challenge. This study aimed to analyze direct and indirect radiation effects on the endothelium by investigating mechanisms of signal transfer from irradiated to nonirradiated endothelial cells by means of secreted proteins. Human coronary artery endothelial cells (HCECest2) undergo radiation-induced senescence in vitro 14 days after exposure to 10 Gy X-rays. Proteomics analysis was performed on HCECest2 14 days after irradiation with X-ray doses of 0 Gy (control) or 10 Gy using label-free technology. Additionally, the proteomes of control and radiation-induced secretomes, and those of nonirradiated HCECest2 exposed for 24 h to secreted proteins of either condition were measured. Key changes identified by proteomics and bioinformatics were validated by immunoblotting, ELISA, bead-based multiplex assays, and targeted transcriptomics. The irradiated cells, their secretome, and the nonirradiated recipient cells showed similar inflammatory response, characterized by induction of interferon type I-related proteins and activation of the STAT3 pathway. These data indicate that irradiated endothelial cells may adversely affect nonirradiated surrounding cells via senescence-associated secretory phenotype. This study adds to our knowledge of the pathological background of radiation-induced cardiovascular disease.

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