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Burwitz, B.J.* ; Wettengel, J.M. ; Mück-Häusl, M. ; Ringelhan, M. ; Ko, C. ; Festag, M.M. ; Hammond, K.B.* ; Northrup, M.* ; Bimber, B.N.* ; Jacob, T.* ; Reed, J.S.* ; Norris, R.* ; Park, B.* ; Moller-Tank, S.* ; Esser, K. ; Greene, J.M.* ; Wu, H.L.* ; Abdulhaqq, S.* ; Webb, G.* ; Sutton, W.F.* ; Klug, A.* ; Swanson, T.* ; Legasse, A.W.* ; Vu, T.Q.* ; Asokan, A.* ; Haigwood, N.L.* ; Protzer, U. ; Sacha, J.B.*

Hepatocytic expression of human sodium-taurocholate cotransporting polypeptide enables hepatitis B virus infection of macaques.

Nat. Commun. 8:2146 (2017)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Hepatitis B virus (HBV) is a major global health concern, and the development of curative therapeutics is urgently needed. Such efforts are impeded by the lack of a physiologically relevant, pre-clinical animal model of HBV infection. Here, we report that expression of the HBV entry receptor, human sodium-taurocholate cotransporting polypeptide (hNTCP), on macaque primary hepatocytes facilitates HBV infection in vitro, where all replicative intermediates including covalently closed circular DNA (cccDNA) are present. Furthermore, viral vector-mediated expression of hNTCP on hepatocytes in vivo renders rhesus macaques permissive to HBV infection. These in vivo macaque HBV infections are characterized by longitudinal HBV DNA in serum, and detection of HBV DNA, RNA, and HBV core antigen (HBcAg) in hepatocytes. Together, these results show that expressing hNTCP on macaque hepatocytes renders them susceptible to HBV infection, thereby establishing a physiologically relevant model of HBV infection to study immune clearance and test therapeutic and curative approaches.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Nonhuman Primate Models; Transgenic Mice; Replication; Vectors; System; Cells; Dna; Genome; Level; Liver
Sprache englisch
Veröffentlichungsjahr 2017
HGF-Berichtsjahr 2017
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 8, Heft: 1, Seiten: , Artikelnummer: 2146 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Virology (VIRO)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-502700-003
DOI 10.1038/s41467-017-01953-y
WOS ID WOS:000418236800008
Scopus ID 85038431583
PubMed ID 29247188
Erfassungsdatum 2017-12-26
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