PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Shah, N. ; Maqbool, M.A.* ; Yahia, Y.* ; El Aabidine, A.Z.* ; Esnault, C.* ; Forné, I.* ; Decker, T.-M. ; Martin, D.* ; Schüller, R. ; Krebs, S.* ; Blum, H.* ; Imhof, A.* ; Eick, D. ; Andrau, J.C.*

Tyrosine-1 of RNA polymerase II CTD controls global termination of gene transcription in mammals.

Mol. Cell 69, 48-61.e6 (2018)
Verlagsversion Postprint Forschungsdaten DOI PMC
Open Access Green
The carboxy-terminal domain (CTD) of RNA polymerase (Pol) II is composed of a repetition of YSPTSPS heptads and functions as a loading platform for protein complexes that regulate transcription, splicing, and maturation of RNAs. Here, we studied mammalian CTD mutants to analyze the function of tyrosine1 residues in the transcription cycle. Mutation of 3/4 of the tyrosine residues (YFFF mutant) resulted in a massive read-through transcription phenotype in the antisense direction of promoters as well as in the 3' direction several hundred kilobases downstream of genes. The YFFF mutant shows reduced Pol II at promoter-proximal pause sites, a loss of interaction with the Mediator and Integrator complexes, and impaired recruitment of these complexes to chromatin. Consistent with these observations, Pol II loading at enhancers and maturation of snRNAs are altered in the YFFF context genome-wide. We conclude that tyrosine1 residues of the CTD control termination of transcription by Pol II.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
14.248
2.782
33
39
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Ctd ; Rna Polymerase Ii ; Tyrosine1 ; Divergent Transcription ; Promoter-proximal Pausing ; Read-through Transcription ; Transcription Termination; Promoter Directionality; Multiprotein Mediator; Kinetic Competition; Factor Recruitment; Mass-spectrometry; Domain; Elongation; Subunit; Polyadenylation; Integrator
Sprache englisch
Veröffentlichungsjahr 2018
HGF-Berichtsjahr 2018
ISSN (print) / ISBN 1097-2765
e-ISSN 1097-4164
Zeitschrift Molecular Cell
Quellenangaben Band: 69, Heft: 1, Seiten: 48-61.e6 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Cambridge
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502890-001
DOI 10.1016/j.molcel.2017.12.009
WOS ID WOS:000419255100007
Scopus ID 85040099657
PubMed ID 29304333
Erfassungsdatum 2018-01-07
[➜Korrektur melden]