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Rehage, N. ; Davydova, E.-O. ; Conrad, C.* ; Behrens, G.* ; Maiser, A.* ; Stehklein, J.E. ; Brenner, S. ; Klein, J.* ; Jeridi, A. ; Hoffmann, A.L.* ; Lee, E.* ; Dianzani, U.* ; Willemsen, R.* ; Feederle, R. ; Reiche, K.* ; Hackermüller, J.* ; Leonhardt, H.* ; Sharma, S.* ; Niessing, D. ; Heissmeyer, V.

Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA.

Nat. Commun. 9:299 (2018)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The ubiquitously expressed RNA-binding proteins Roquin-1 and Roquin-2 are essential for appropriate immune cell function and postnatal survival of mice. Roquin proteins repress target mRNAs by recognizing secondary structures in their 3'-UTRs and by inducing mRNA decay. However, it is unknown if other cellular proteins contribute to target control. To identify cofactors of Roquin, we used RNA interference to screen similar to 1500 genes involved in RNA-binding or mRNA degradation, and identified NUFIP2 as a cofactor of Roquin-induced mRNA decay. NUFIP2 binds directly and with high affinity to Roquin, which stabilizes NUFIP2 in cells. Post-transcriptional repression of human ICOS by endogenous Roquin proteins requires two neighboring non-canonical stem-loops in the ICOS 3'-UTR. This unconventional cis-element as well as another tandem loop known to confer Roquin-mediated regulation of the Ox40 3'-UTR, are bound cooperatively by Roquin and NUFIP2. NUFIP2 therefore emerges as a cofactor that contributes to mRNA target recognition by Roquin.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Constitutive-decay Element; Mental-retardation Protein; Tfh-cell-differentiation; Helper T-cells; Inducible Costimulator; Dependent Decay; Stress Granule; Stem-loop; B-cells; Autoimmunity
Sprache englisch
Veröffentlichungsjahr 2018
HGF-Berichtsjahr 2018
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 9, Heft: 1, Seiten: , Artikelnummer: 299 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Molecular Immune Regulation (AMIR)
Institute of Structural Biology (STB)
CF Monoclonal Antibodies (CF-MAB)
POF Topic(s) 30203 - Molecular Targets and Therapies
30201 - Metabolic Health
Forschungsfeld(er) Immune Response and Infection
Enabling and Novel Technologies
Helmholtz Diabetes Center
PSP-Element(e) G-501712-001
G-503091-001
G-502210-001
DOI 10.1038/s41467-017-02582-1
WOS ID WOS:000422911900003
Scopus ID 85040949581
PubMed ID 29352114
Erfassungsdatum 2018-02-28
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