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Sigmund, F. ; Massner, C. ; Erdmann, P.* ; Stelzl, A. ; Rolbieski, H. ; Desai, M.* ; Bricault, S.* ; Wörner, T.P.* ; Snijder, J.* ; Geerlof, A. ; Fuchs, H. ; Hrabě de Angelis, M. ; Heck, A.J.R.* ; Jasanoff, A.* ; Ntziachristos, V. ; Plitzko, J.* ; Westmeyer, G.G.

Bacterial encapsulins as orthogonal compartments for mammalian cell engineering.

Nat. Commun. 9:1990 (2018)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
We genetically controlled compartmentalization in eukaryotic cells by heterologous expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic reactions and size-constrained metal biomineralization. The shell protein (EncA) from Myxococcus xanthus auto-assembles into nanocompartments inside mammalian cells to which sets of native (EncB,C,D) and engineered cargo proteins self-target enabling localized bimolecular fluorescence and enzyme complementation. Encapsulation of the enzyme tyrosinase leads to the confinement of toxic melanin production for robust detection via multispectral optoacoustic tomography (MSOT). Co-expression of ferritin-like native cargo (EncB,C) results in efficient iron sequestration producing substantial contrast by magnetic resonance imaging (MRI) and allowing for magnetic cell sorting. The monodisperse, spherical, and iron-loading nanoshells are also excellent genetically encoded reporters for electron microscopy (EM). In general, eukaryotically expressed encapsulins enable cellular engineering of spatially confined multicomponent processes with versatile applications in multiscale molecular imaging, as well as intriguing implications for metabolic engineering and cellular therapy.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 2018
HGF-Berichtsjahr 2018
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 9, Heft: 1, Seiten: , Artikelnummer: 1990 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Institute of Structural Biology (STB)
Institute of Experimental Genetics (IEG)
Institute of Developmental Genetics (IDG)
POF Topic(s) 30505 - New Technologies for Biomedical Discoveries
30205 - Bioengineering and Digital Health
30203 - Molecular Targets and Therapies
30201 - Metabolic Health
30204 - Cell Programming and Repair
Forschungsfeld(er) Enabling and Novel Technologies
Genetics and Epidemiology
PSP-Element(e) G-552000-001
G-505592-001
G-503000-001
G-500600-001
G-505500-001
G-500500-001
DOI 10.1038/s41467-018-04227-3
WOS ID WOS:000432444100004
Scopus ID 85047194244
PubMed ID 29777103
Erfassungsdatum 2018-05-22
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