PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Bouchard, C.* ; Sahu, P.* ; Meixner, M.* ; Nötzold, R.R.* ; Rust, M.B.* ; Kremmer, E. ; Feederle, R. ; Hart-Smith, G.* ; Finkernagel, F.* ; Bartkuhn, M.* ; Savai Pullamsetti, S.* ; Nist, A.* ; Stiewe, T.* ; Philipsen, S.* ; Bauer, U.M.*

Genomic location of PRMT6-dependent H3R2 methylation is linked to the transcriptional outcome of associated genes.

Cell Rep. 24, 3339-3352 (2018)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Protein arginine methyltransferase 6 (PRMT6) catalyzes asymmetric dimethylation of histone H3 at arginine 2 (H3R2me2a). This mark has been reported to associate with silent genes. Here, we use a cell model of neural differentiation, which upon PRMT6 knockout exhibits proliferation and differentiation defects. Strikingly, we detect PRMT6-dependent H3R2me2a at active genes, both at promoter and enhancer sites. Loss of H3R2me2a from promoter sites leads to enhanced KMT2A binding and H3K4me3 deposition together with increased target gene transcription, supporting a repressive nature of H3R2me2a. At enhancers, H3R2me2a peaks co-localize with the active enhancer marks H3K4me1 and H3K27ac. Here, loss of H3R2me2a results in reduced KMT2D binding and H3K4me1/H3K27ac deposition together with decreased transcription of associated genes, indicating that H3R2me2a also exerts activation functions. Our work suggests that PRMT6 via H3R2me2a interferes with the deposition of adjacent histone marks and modulates the activity of important differentiation-associated genes by opposing transcriptional effects.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
8.032
1.648
13
25
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Chromatin ; Gene Expression ; Histone Arginine Methylation ; Histone Code ; Histone Modifications ; Neural Differentiation ; Pluripotency ; Posttranslational Modifications ; Protein Arginine Methyltransferases ; Transcriptional Regulation; Histone Arginine Methylation; Embryonal Carcinoma-cells; H3k4 Trimethylation; Tumor-suppressor; Prmt6; Expression; Differentiation; Repression; Senescence; Identity
Sprache englisch
Veröffentlichungsjahr 2018
HGF-Berichtsjahr 2018
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Zeitschrift Cell Reports
Quellenangaben Band: 24, Heft: 12, Seiten: 3339-3352 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Immunology (IMI)
CF Monoclonal Antibodies (CF-MAB)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30201 - Metabolic Health
Forschungsfeld(er) Immune Response and Infection
Helmholtz Diabetes Center
PSP-Element(e) G-501793-001
G-502210-001
DOI 10.1016/j.celrep.2018.08.052
WOS ID WOS:000444924100022
Scopus ID 85053036235
PubMed ID 30232013
Erfassungsdatum 2018-09-26
[➜Korrektur melden]