PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Kreyling, W.G. ; Möller, W. ; Holzwarth, U.* ; Hirn, S. ; Wenk, A. ; Schleh, C. ; Schäffler, M. ; Haberl, N. ; Gibson, N.* ; Schittny, J.C.*

Age-dependent rat lung deposition patterns of inhaled 20 manometer gold nanoparticles and their quantitative biokinetics in adult rats.

ACS Nano 12, 7771-7790 (2018)
Verlagsversion Forschungsdaten DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
The increasing use of gold nanoparticles leads to a possible increase of exposure by inhalation. Therefore, we have studied the deposition patterns of inhaled 20 nm gold nanoparticles (AuNP) in 7-90 day old rats and their biokinetics in 60 day old ones. Wistar-Kyoto rats inhaled intratracheally 20 nm Au-195-radiolabeled AuNP by negative pressure ventilation over 2 h. Immediately afterward lungs were excised, inflated and microwave dried. AuNP deposition was analyzed by single-photon emission computed tomography, computed-tomography and autoradiography. Completely balanced, quantitative biodistributions in major organs and all body tissues and total excretion were analyzed from 1 h to 28 d after inhalation. Intratracheal inhalation caused AuNP deposition predominately in the caudal lungs, independent of age. About 30% AuNP were deposited on airway epithelia and rapidly cleared by mucociliary clearance. About 80% of AuNP deposited in alveoli was relocated from the epithelium into the interstitium within 24 h and was inaccessible to broncho-alveolar lavage. During interstitial long-term retention, re-entrainment within macrophages back onto the lung epithelium and to the larynx and gastrointestinal tract (GIT) dominated AuNP clearance (rate 0.03 d(-1)) In contrast, AuNP-translocation across the air-blood barrier was much smaller leading to persistent retention in secondary organs and tissues in the ranking order liver > soft issue > spleen > kidneys > skeleton > blood > uterus > heart > brain. The age independent, inhomogeneous AuNP deposition was probably caused by the negative pressure ventilation. Long-term AuNP clearance was dominated by macrophage-mediated transport from the interstitium to the larynx and GIT. Translocation across the rat air blood barrier appeared to be similar to that of humans for similar sized AuNP.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
13.709
2.580
29
48
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Gold Nanoparticle Aerosols ; Intratracheal Inhalation ; Air-blood Barrier Translocation ; Extrapulmonary Nanoparticle Accumulation ; Nanoparticle Clearance ; Alveolar Macrophage ; Interstitial Nanoparticle Relocation ; Epithelial Re-entrainment; Titanium-dioxide Nanoparticles; Pulmonary Macrophagic System; Insoluble Iridium Particles; Long-term Clearance; Ultrafine Particles; In-vivo; Alveolar Macrophages; Acinar Structures; Secondary Organs; Renal Clearance
Sprache englisch
Veröffentlichungsjahr 2018
HGF-Berichtsjahr 2018
ISSN (print) / ISBN 1936-0851
e-ISSN 1936-086X
Zeitschrift ACS Nano
Quellenangaben Band: 12, Heft: 8, Seiten: 7771-7790 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort 1155 16th St, Nw, Washington, Dc 20036 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Epidemiology (EPI)
Institute of Lung Health and Immunity (LHI)
POF Topic(s) 30202 - Environmental Health
Forschungsfeld(er) Genetics and Epidemiology
Lung Research
PSP-Element(e) G-504000-001
G-505000-001
DOI 10.1021/acsnano.8b01826
WOS ID WOS:000443525600031
PubMed ID 30085651
Erfassungsdatum 2018-09-24
[➜Korrektur melden]