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Rohrmoser, M. ; Kluge, M.* ; Yahia, Y.* ; Gruber-Eber, A. ; Maqbool, M.A.* ; Forné, I.* ; Krebs, S.* ; Blum, H.* ; Greifenberg, A.K.* ; Geyer, M.* ; Descostes, N.* ; Imhof, A.* ; Andrau, J.C.* ; Friedel, C.C.* ; Eick, D.

MIR sequences recruit zinc finger protein ZNF768 to expressed genes.

Nucleic Acids Res. 47, 700-715 (2019)
Verlagsversion Forschungsdaten DOI PMC
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Creative Commons Lizenzvertrag
Mammalian-wide interspersed repeats (MIRs) are retrotransposed elements of mammalian genomes. Here, we report the specific binding of zinc finger protein ZNF768 to the sequence motif GCTGTGTG (N-20) CCTCTCTG in the core region of MIRs. ZNF768 binding is preferentially associated with euchromatin and promoter regions of genes. Binding was observed for genes expressed in a cell type-specific manner in human B cell line Raji and osteosarcoma U2OS cells. Mass spectrometric analysis revealed binding of ZNF768 to Elongator components Elp1, Elp2 and Elp3 and other nuclear factors. The N-terminus of ZNF768 contains a heptad repeat array structurally related to the C-terminal domain (CTD) of RNA polymerase II. This array evolved in placental animals but not marsupials and monotreme species, displays species-specific length variations, and possibly fulfills CTD related functions in gene regulation. We propose that the evolution of MIRs and ZNF768 has extended the repertoire of gene regulatory mechanisms in mammals and that ZNF768 binding is associated with cell type-specific gene expression.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Rna-polymerase-ii; C-terminal Domain; Ctd; Transcription; Evolution; Phosphorylation; Complexity; Elements; Package; Binding
Sprache
Veröffentlichungsjahr 2019
Prepublished im Jahr 2018
HGF-Berichtsjahr 2018
ISSN (print) / ISBN 0305-1048
e-ISSN 1362-4962
Zeitschrift Nucleic Acids Research
Quellenangaben Band: 47, Heft: 2, Seiten: 700-715 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Verlagsort Great Clarendon St, Oxford Ox2 6dp, England
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502890-001
DOI 10.1093/nar/gky1148
WOS ID WOS:000467959100020
Scopus ID 85060581320
PubMed ID 30476274
Erfassungsdatum 2018-12-07
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