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Heber, S. ; Gáspár, I.* ; Tants, J.-N. ; Günther, J. ; Fernandez Moya, S.M.* ; Janowski, R. ; Ephrussi, A.* ; Sattler, M. ; Niessing, D.

Staufen2-mediated RNA recognition and localization requires combinatorial action of multiple domains.

Nat. Commun. 10:1659 (2019)
Verlagsversion Forschungsdaten DOI PMC
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Throughout metazoans, Staufen (Stau) proteins are core factors of mRNA localization particles. They consist of three to four double-stranded RNA binding domains (dsRBDs) and a Cterminal dsRBD-like domain. Mouse Staufen2 (mStau2)-like Drosophila Stau (dmStau) contains four dsRBDs. Existing data suggest that only dsRBDs 3-4 are necessary and sufficient for mRNA binding. Here, we show that dsRBDs 1 and 2 of mStau2 bind RNA with similar affinities and kinetics as dsRBDs 3 and 4. While RNA binding by these tandem domains is transient, all four dsRBDs recognize their target RNAs with high stability. Rescue experiments in Drosophila oocytes demonstrate that mStau2 partially rescues dmStau-dependent mRNA localization. In contrast, a rescue with mStau2 bearing RNA-binding mutations in dsRBD1-2 fails, confirming the physiological relevance of our findings. In summary, our data show that the dsRBDs 1-2 play essential roles in the mRNA recognition and function of Stau-family proteins of different species.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Double-stranded-rna; Binding-protein Staufen2; Somatodendritic Domain; Secondary Structures; Mammalian Staufen; Nmr; Segregation; Platform; Distinct; Neurons
Sprache englisch
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 10, Heft: 1, Seiten: , Artikelnummer: 1659 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503091-001
G-503000-001
DOI 10.1038/s41467-019-09655-3
WOS ID WOS:000463984400008
Scopus ID 85064721418
PubMed ID 30971701
Erfassungsdatum 2019-04-11
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