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Abd Rahim, M.S.* ; Cherniavskyi, Y.K.* ; Tieleman, D.P.* ; Dames, S.A.

NMR- and MD simulation-based structural characterization of the membrane-associating FATC domain of ataxia telangiectasia mutated.

J. Biol. Chem. 294, 7098-7112 (2019)
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Creative Commons Lizenzvertrag
The Ser/Thr protein kinase ataxia telangiectasia mutated (ATM) plays an important role in the DNA damage response, signaling in response to redox signals, the control of metabolic processes, and mitochondrial homeostasis. ATM localizes to the nucleus and at the plasma membrane, mitochondria, peroxisomes, and other cytoplasmic vesicular structures. It has been shown that the C-terminal FATC domain of human ATM (hATMfatc) can interact with a range of membrane mimetics and may thereby act as a membrane-anchoring unit. Here, NMR structural and N-15 relaxation data, NMR data using spin-labeled micelles, and MD simulations of micelle-associated hATMfatc revealed that it binds the micelle by a dynamic assembly of three helices with many residues of hATMfatc located in the headgroup region. We observed that none of the three helices penetrates the micelle deeply or makes significant tertiary contacts to the other helices. NMR-monitored interaction experiments with hATMfatc variants in which two conserved aromatic residues (Phe(3049) and Trp(3052)) were either individually or both replaced by alanine disclosed that the double substitution does not abrogate the interaction with micelles and bicelles at the high concentrations at which these aggregates are typically used, but impairs interactions with small unilamellar vesicles, usually used at much lower lipid concentrations and considered a better mimetic for natural membranes. We conclude that the observed dynamic structure of micelle-associated hATMfatc may enable it to interact with differently composed membranes or membrane-associated interaction partners and thereby regulate ATM's kinase activity. Moreover, the FATC domain of ATM may function as a membrane-anchoring unit for other biomolecules.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Protein Kinase ; Membrane ; Nuclear Magnetic Resonance (nmr) ; Molecular Dynamics (md) Simulation ; Signal Transduction ; Ataxia Telangiectasia Mutated ; Phosphatidylinositol 3-kinase-related Kinase ; Protein Membrane Interactions ; Signaling At Membranes ; Structural Dynamics ; Fatc Domain ; Solution Structure; Phosphatidylinositol 3-kinase-related Kinases; Particle Mesh Ewald; Atm Protein-kinase; Cellular-response; Gene-product; Dna-pkcs; Rapamycin; Target; Peptides; Activation
Sprache
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 0021-9258
e-ISSN 1083-351X
Zeitschrift Journal of Biological Chemistry, The
Quellenangaben Band: 294, Heft: 17, Seiten: 7098-7112 Artikelnummer: , Supplement: ,
Verlag American Society for Biochemistry and Molecular Biology
Verlagsort 9650 Rockville Pike, Bethesda, Md 20814-3996 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503000-001
DOI 10.1074/jbc.RA119.007653
WOS ID WOS:000467394700040
Scopus ID 85065097418
Erfassungsdatum 2019-05-14
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