PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Bastidas-Ponce, A. ; Tritschler, S. ; Dony, L. ; Scheibner, K. ; Tarquis-Medina, M. ; Salinno, C. ; Schirge, S. ; Burtscher, I. ; Böttcher, A. ; Theis, F.J. ; Lickert, H. ; Bakhti, M.

Comprehensive single cell mRNA profiling reveals a detailed roadmap for pancreatic endocrinogenesis.

Development 146:dev173849 (2019)
Verlagsversion Postprint Forschungsdaten DOI PMC
Open Access Green
Deciphering mechanisms of endocrine cell induction, specification and lineage allocation in vivo will provide valuable insights into how the islets of Langerhans are generated. Currently, it is ill defined how endocrine progenitors segregate into different endocrine subtypes during development. Here, we generated a novel neurogenin 3 (Ngn3)-Venus fusion (NVF) reporter mouse line, that closely mirrors the transient endogenous Ngn3 protein expression. To define an in vivo roadmap of endocrinogenesis, we performed single cell RNA sequencing of 36,351 pancreatic epithelial and NVF+ cells during secondary transition. This allowed Ngn3(low) endocrine progenitors, Ngn3(high) endocrine precursors, Fev(+) endocrine lineage and hormone(+) endocrine subtypes to be distinguished and time-resolved, and molecular programs during the step-wise lineage restriction steps to be delineated. Strikingly, we identified 58 novel signature genes that show the same transient expression dynamics as Ngn3 in the 7260 profiled Ngn3-expressing cells. The differential expression of these genes in endocrine precursors associated with their cell-fate allocation towards distinct endocrine cell types. Thus, the generation of an accurately regulated NVF reporter allowed us to temporally resolve endocrine lineage development to provide a fine-grained single cell molecular profile of endocrinogenesis in vivo.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
5.763
1.453
30
59
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Endocrine Progenitor-precursor ; Neurog3 ; Single Cell Rna Sequencing ; Endocrinogenesis ; Endocrine Cell Allocation ; Mouse; Endocrine Progenitors; Differentiation; Mouse; Neurogenin3; Organogenesis; Expression; Phosphorylation; Maintenance; Lineage; Plexus
Sprache englisch
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 0950-1991
e-ISSN 1477-9129
Zeitschrift Development / Company of Biologists
Quellenangaben Band: 146, Heft: 12, Seiten: , Artikelnummer: dev173849 Supplement: ,
Verlag Company of Biologists
Verlagsort Bidder Building, Station Rd, Histon, Cambridge Cb24 9lf, England
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Regeneration Research (IDR)
Institute of Computational Biology (ICB)
POF Topic(s) 30201 - Metabolic Health
30205 - Bioengineering and Digital Health
90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
Enabling and Novel Technologies
PSP-Element(e) G-502300-001
G-503800-001
G-501900-231
DOI 10.1242/dev.173849
WOS ID WOS:000473330400018
PubMed ID 31160421
Erfassungsdatum 2019-06-06
[➜Korrektur melden]