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Meier, S.* ; Bohnacker, S. ; Klose, C.J.* ; Lopez, A. ; Choe, C.A.* ; Schmid, P.W.N.* ; Bloemeke, N.* ; Rührnößl, F.* ; Haslbeck, M.* ; Esser-von Bieren, J.* ; Sattler, M. ; Huang, P.S.* ; Feige, M.J.*

The molecular basis of chaperone-mediated interleukin 23 assembly control.

Nat. Commun. 10:4121 (2019)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
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The functionality of most secreted proteins depends on their assembly into a defined quaternary structure. Despite this, it remains unclear how cells discriminate unassembled proteins en route to the native state from misfolded ones that need to be degraded. Here we show how chaperones can regulate and control assembly of heterodimeric proteins, using interleukin 23 (IL-23) as a model. We find that the IL-23 α-subunit remains partially unstructured until assembly with its β-subunit occurs and identify a major site of incomplete folding. Incomplete folding is recognized by different chaperones along the secretory pathway, realizing reliable assembly control by sequential checkpoints. Structural optimization of the chaperone recognition site allows it to bypass quality control checkpoints and provides a secretion-competent IL-23α subunit, which can still form functional heterodimeric IL-23. Thus, locally-restricted incomplete folding within single-domain proteins can be used to regulate and control their assembly.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 10, Heft: 1, Seiten: , Artikelnummer: 4121 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)
Institute of Structural Biology (STB)
POF Topic(s) 30202 - Environmental Health
30203 - Molecular Targets and Therapies
Forschungsfeld(er) Allergy
Enabling and Novel Technologies
PSP-Element(e) G-554600-001
G-503000-001
DOI 10.1038/s41467-019-12006-x
Scopus ID 85072119135
PubMed ID 31511508
Erfassungsdatum 2019-10-02
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