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Binger, K.J.* ; Neukam, M. ; Tattikota, S.G.* ; Qadri, F.* ; Puchkov, D.* ; Willmes, D.M. ; Wurmsee, S.* ; Geisberger, S.* ; Dechend, R.* ; Raile, K.* ; Kurth, T.* ; Nguyen, G.* ; Poy, M.N.* ; Solimena, M. ; Müller, D.N.* ; Birkenfeld, A.L.

Atp6ap2 deletion causes extensive vacuolation that consumes the insulin content of pancreatic β cells.

Proc. Natl. Acad. Sci. U.S.A. 116, 19983-19988 (2019)
Verlagsversion DOI PMC
Open Access Gold
Pancreatic β cells store insulin within secretory granules which undergo exocytosis upon elevation of blood glucose levels. Crinophagy and autophagy are instead responsible to deliver damaged or old granules to acidic lysosomes for intracellular degradation. However, excessive consumption of insulin granules can impair β cell function and cause diabetes. Atp6ap2 is an essential accessory component of the vacuolar ATPase required for lysosomal degradative functions and autophagy. Here, we show that Cre recombinase-mediated conditional deletion of Atp6ap2 in mouse β cells causes a dramatic accumulation of large, multigranular vacuoles in the cytoplasm, with reduction of insulin content and compromised glucose homeostasis. Loss of insulin stores and gigantic vacuoles were also observed in cultured insulinoma INS-1 cells upon CRISPR/Cas9-mediated removal of Atp6ap2. Remarkably, these phenotypic alterations could not be attributed to a deficiency in autophagy or acidification of lysosomes. Together, these data indicate that Atp6ap2 is critical for regulating the stored insulin pool and that a balanced regulation of granule turnover is key to maintaining β cell function and diabetes prevention.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter (pro)renin Receptor ; Autophagy ; Diabetes ; Vacuolar H Atpase +
Sprache englisch
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Zeitschrift Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Band: 116, Heft: 40, Seiten: 19983-19988 Artikelnummer: , Supplement: ,
Verlag National Academy of Sciences
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502600-012
G-502600-001
DOI 10.1073/pnas.1903678116
Scopus ID 85072758913
PubMed ID 31527264
Erfassungsdatum 2019-09-26
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