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Zak, K.M. ; Kalińska, M.* ; Wątor, E.* ; Kuśka, K.* ; Krutyhołowa, R.* ; Dubin, G.* ; Popowicz, G.M. ; Grudnik, P.*

Crystal structure of kluyveromyces lactis glucokinase (KlGlk1).

Int. J. Mol. Sci. 20:4821 (2019)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Glucose phosphorylating enzymes are crucial in the regulation of basic cellular processes, including metabolism and gene expression. Glucokinases and hexokinases provide a pool of phosphorylated glucose in an adenosine diphosphate (ADP)- and ATP-dependent manner to shape the cell metabolism. The glucose processing enzymes from Kluyveromyces lactis are poorly characterized despite the emerging contribution of this yeast strain to industrial and laboratory scale biotechnology. The first reports on K. lactis glucokinase (KlGlk1) positioned the enzyme as an essential component required for glucose signaling. Nevertheless, no biochemical and structural information was available until now. Here, we present the first crystal structure of KlGlk1 together with biochemical characterization, including substrate specificity and enzyme kinetics. Additionally, comparative analysis of the presented structure and the prior structures of lactis hexokinase (KlHxk1) demonstrates the potential transitions between open and closed enzyme conformations upon ligand binding.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Kluyveromyces Lactis ; Glucokinase ; Sugar Metabolism; Adp-dependent Glucokinase; Hexokinase Pii; Glucose; Protein; Yeast; Complex; Enzyme; Optimization; Repression; Resolution
Sprache
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 1661-6596
e-ISSN 1422-0067
Zeitschrift International Journal of Molecular Sciences
Quellenangaben Band: 20, Heft: 19, Seiten: , Artikelnummer: 4821 Supplement: ,
Verlag MDPI
Verlagsort Basel
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503000-001
DOI 10.3390/ijms20194821
WOS ID WOS:000494798300162
Scopus ID 85072762308
PubMed ID 31569356
Erfassungsdatum 2019-10-07
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