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Pauly, D.* ; Agarwal, D.* ; Dana, N.* ; Schäfer, N.* ; Biber, J.* ; Wunderlich, K.A.* ; Jabri, Y.* ; Straub, T.* ; Zhang, N.R.* ; Gautam, A.K.* ; Weber, B.H.F.* ; Hauck, S.M. ; Kim, M.* ; Curcio, C.A.* ; Stambolian, D.* ; Li, M.* ; Grosche, A.*

Cell type-specific complement expression in the healthy and diseased retina.

Cell Rep. 29, 2835-2848.e4 (2019)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Complement dysregulation is a feature of many retinal diseases, yet mechanistic understanding at the cellular level is limited. Given this knowledge gap about which retinal cells express complement, we performed single-cell RNA sequencing on similar to 92,000 mouse retinal cells and validated our results in five major purified retinal cell types. We found evidence for a distributed cell-type-specific complement expression across 11 cell types. Notably, Muller cells are the major contributor of complement activators c1s, c3, c4, and cfb. Retinal pigment epithelium (RPE) mainly expresses cfh and the terminal complement components, whereas cfi and cfp transcripts are most abundant in neurons. Aging enhances c1s, cfb, cfp, and cfi expression, while cfh expression decreases. Transient retinal ischemia increases complement expression in microglia, Muller cells, and RPE. In summary, we report a unique complement expression signature for murine retinal cell types suggesting a well-orchestrated regulation of local complement expression in the retinal microenvironment.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Age-dependent ; Alternative ; Classical ; Complement Expression ; Eye ; Ischemia ; Lectin ; Müller Cell ; Neuron ; Retina; Gene-expression; Pigment Epithelium; Glial-cells; Mouse Model; Factor-i; Activation; System
Sprache englisch
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Zeitschrift Cell Reports
Quellenangaben Band: 29, Heft: 9, Seiten: 2835-2848.e4 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-505700-001
A-630700-001
DOI 10.1016/j.celrep.2019.10.084
WOS ID WOS:000499377000027
Scopus ID 85075515258
PubMed ID 31775049
Erfassungsdatum 2019-12-04
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