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Darr, J. ; Tomar, A. ; Lassi, M. ; Gerlini, R. ; Berti, L. ; Hering, A. ; Scheid, F. ; Hrabě de Angelis, M. ; Witting, M. ; Teperino, R.

iTAG-RNA isolates cell-specific transcriptional responses to environmental stimuli and identifies an RNA-based endocrine axis.

Cell Rep. 30, 3183-3194.e4 (2020)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Biofluids contain various circulating cell-free RNAs (ccfRNAs). The composition of these ccfRNAs varies among biofluids. They constitute tantalizing biomarker candidates for several pathologies and have been demonstrated to be mediators of cellular communication. Little is known about their function in physiological and developmental settings, and most works are limited to in vitro studies. Here, we develop iTAG-RNA, a method for the unbiased tagging of RNA transcripts in mice in vivo. We use iTAG-RNA to isolate hepatocytes and kidney proximal epithelial cell-specific transcriptional responses to a dietary challenge without interfering with the tissue architecture and to identify multiple hepatocyte-secreted ccfRNAs in plasma. We also identify specific transfer of liver-derived ccfRNAs to adipose tissue and skeletal muscle, where they likely constitute a buffering system to maintain lipid homeostasis under acute high-fat-diet feeding. Our findings directly demonstrate in vivo transfer of RNAs between tissues and highlight its implications for endocrine signaling and homeostasis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Rna Tagging, Mouse Genetics, Circulating Rna, 5eu Prodrug, Epigenetics, Rna Biomarker; Proliferator-activated Receptor; Click Chemistry; Microrna 122; Expression; Liver; Gene; Inflammation; Interference; Cholesterol; Prodrug
Sprache englisch
Veröffentlichungsjahr 2020
HGF-Berichtsjahr 2020
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Zeitschrift Cell Reports
Quellenangaben Band: 30, Heft: 9, Seiten: 3183-3194.e4 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Experimental Genetics (IEG)
Institute of Diabetes Research and Metabolic Diseases (IDM)
Research Unit BioGeoChemistry and Analytics (BGC)
POF Topic(s) 90000 - German Center for Diabetes Research
30201 - Metabolic Health
30202 - Environmental Health
Forschungsfeld(er) Genetics and Epidemiology
Helmholtz Diabetes Center
Environmental Sciences
PSP-Element(e) G-501900-069
G-500692-001
G-502400-001
G-501900-065
G-500600-001
G-504800-001
DOI 10.1016/j.celrep.2020.02.020
WOS ID WOS:000517773300027
Scopus ID 85080118995
PubMed ID 32130917
Erfassungsdatum 2020-03-11
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