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Burbulla, L.F.* ; Schelling, C.* ; Kato, H.* ; Rapaport, D.* ; Woitalla, D.* ; Schiesling, C.* ; Schulte, C.* ; Sharma, M.* ; Illig, T. ; Bauer, P.* ; Jung, S.* ; Nordheim, A.* ; Schöls, L.* ; Riess, O.* ; Kruger, R.*

Dissecting the role of the mitochondrial chaperone mortalin in Parkinson's disease: Functional impact of disease-related variants on mitochondrial homeostasis.

Hum. Mol. Genet. 19, 4437-4452 (2010)
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The mitochondrial chaperone mortalin has been linked to neurodegeneration in Parkinson's disease (PD) based on reduced protein levels in affected brain regions of PD patients and its interaction with the PD-associated protein DJ-1. Recently, two amino acid exchanges in the ATPase domain (R126W) and the substrate-binding domain (P509S) of mortalin were identified in Spanish PD patients. Here, we identified a separate and novel variant (A476T) in the substrate-binding domain of mortalin in German PD patients. To define a potential role as a susceptibility factor in PD, we characterized the functions of all three variants in different cellular models. In vitro import assays revealed normal targeting of all mortalin variants. In neuronal and non-neuronal human cell lines, the disease-associated variants caused a mitochondrial phenotype of increased reactive oxygen species and reduced mitochondrial membrane potential, which were exacerbated upon proteolytic stress. These functional impairments correspond with characteristic alterations of the mitochondrial network in cells overexpressing mutant mortalin compared with wild-type (wt), which were confirmed in fibroblasts from a carrier of the A476T variant. In line with a loss of function hypothesis, knockdown of mortalin in human cells caused impaired mitochondrial function that was rescued by wt mortalin, but not by the variants. Our genetic and functional studies of novel disease-associated variants in the mortalin gene define a loss of mortalin function, which causes impaired mitochondrial function and dynamics. Our results support the role of this mitochondrial chaperone in neurodegeneration and underscore the concept of impaired mitochondrial protein quality control in PD.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter EARLY-ONSET PARKINSONISM; OXIDATIVE STRESS; LINKAGE ANALYSIS; PROTEIN; DJ-1; GENE; MUTATIONS; IMPORT; HSP70; IDENTIFICATION; EARLY-ONSET PARKINSONISM; OXIDATIVE STRESS; LINKAGE ANALYSIS; PROTEIN; GENE; DJ-1; MUTATIONS; IMPORT; HSP70; IDENTIFICATION
Sprache englisch
Veröffentlichungsjahr 2010
HGF-Berichtsjahr 2010
ISSN (print) / ISBN 0964-6906
e-ISSN 1460-2083
Zeitschrift Human Molecular Genetics
Quellenangaben Band: 19, Heft: 22, Seiten: 4437-4452 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Epidemiology (EPI)
POF Topic(s) 30503 - Chronic Diseases of the Lung and Allergies
Forschungsfeld(er) Genetics and Epidemiology
PSP-Element(e) G-503900-003
PubMed ID 20817635
DOI 10.1093/hmg/ddq370
WOS ID WOS:000283673500013
Scopus ID 77958479016
DNB ID 835/2010
Erfassungsdatum 2010-12-31
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