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Santos-Rosa, H.* ; Millán-Zambrano, G.* ; Han, N.* ; Leonardi, T.* ; Klimontova, M.* ; Nasiscionyte, S. ; Pandolfini, L.* ; Tzelepis, K.* ; Bartke, T. ; Kouzarides, T.*

Methylation of histone H3 at lysine 37 by Set1 and Set2 prevents spurious DNA replication.

Mol. Cell 81, 2793-2807.e8 (2021)
Postprint Forschungsdaten DOI PMC
Open Access Green
DNA replication initiates at genomic locations known as origins of replication, which, in S. cerevisiae, share a common DNA consensus motif. Despite being virtually nucleosome-free, origins of replication are greatly influenced by the surrounding chromatin state. Here, we show that histone H3 lysine 37 mono-methylation (H3K37me1) is catalyzed by Set1p and Set2p and that it regulates replication origin licensing. H3K37me1 is uniformly distributed throughout most of the genome, but it is scarce at replication origins, where it increases according to the timing of their firing. We find that H3K37me1 hinders Mcm2 interaction with chromatin, maintaining low levels of MCM outside of conventional replication origins. Lack of H3K37me1 results in defective DNA replication from canonical origins while promoting replication events at inefficient and non-canonical sites. Collectively, our results indicate that H3K37me1 ensures correct execution of the DNA replication program by protecting the genome from inappropriate origin licensing and spurious DNA replication.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter H3k37methylation ; Histone Modifications ; Mcm ; Origin Licensing ; Replication Origins ; Set1 ; Set2; Compass Family; Origin; Initiation; Proteins; Yeast; Acetylation; Chromatin; Program; Methyltransferase; Reveals
Sprache englisch
Veröffentlichungsjahr 2021
HGF-Berichtsjahr 2021
ISSN (print) / ISBN 1097-2765
e-ISSN 1097-4164
Zeitschrift Molecular Cell
Quellenangaben Band: 81, Heft: 13, Seiten: 2793-2807.e8 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502800-001
Förderungen Helmholtz Association
European Research Council (ERC)
Sir Henry Wellcome Fellowship
Asociacion Espanola Contra el Cancer
European Molecular Biology Organization (EMBO)
Wellcome Trust
Cancer Research UK
DOI 10.1016/j.molcel.2021.04.021
WOS ID WOS:000672572800012
Scopus ID 85108964189
PubMed ID 33979575
Erfassungsdatum 2021-06-18
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