PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Chanou, A. ; Hamperl, S.

Single-molecule techniques to study chromatin.

Front. Cell Dev. Biol. 9:699771 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Besides the basic organization in nucleosome core particles (NCPs), eukaryotic chromatin is further packed through interactions with numerous protein complexes including transcription factors, chromatin remodeling and modifying enzymes. This nucleoprotein complex provides the template for many important biological processes, such as DNA replication, transcription, and DNA repair. Thus, to understand the molecular basis of these DNA transactions, it is critical to define individual changes of the chromatin structure at precise genomic regions where these machineries assemble and drive biological reactions. Single-molecule approaches provide the only possible solution to overcome the heterogenous nature of chromatin and monitor the behavior of individual chromatin transactions in real-time. In this review, we will give an overview of currently available single-molecule methods to obtain mechanistic insights into nucleosome positioning, histone modifications and DNA replication and transcription analysis-previously unattainable with population-based assays.
Impact Factor
Scopus SNIP
Scopus
Cited By
Altmetric
6.684
1.283
5
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Schlagwörter Dna Fiber Analysis ; Chromatin Accessibility ; Chromatin Replication/transcription ; Electron Microscopy ; Histone Modifications ; Magnetic/optical Tweezers ; Single-molecule Techniques ; Third-generation Sequencing; Rna-polymerase-ii; Lagging-strand Synthesis; Dna-replication; In-vivo; Transcription Initiation; Nucleosomal Barrier; Fork Reversal; Cross-linking; Human Genome; Cell-cycle
Sprache englisch
Veröffentlichungsjahr 2021
HGF-Berichtsjahr 2021
ISSN (print) / ISBN 2296-634X
e-ISSN 2296-634X
Zeitschrift Frontiers in cell and developmental biology
Quellenangaben Band: 9, Heft: , Seiten: , Artikelnummer: 699771 Supplement: ,
Verlag Frontiers
Verlagsort Lausanne
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Epigenetics and Stem Cells (IES)
POF Topic(s) 30204 - Cell Programming and Repair
Forschungsfeld(er) Stem Cell and Neuroscience
PSP-Element(e) G-554500-001
Förderungen European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program
Helmholtz Association
DOI 10.3389/fcell.2021.699771
WOS ID WOS:000674263200001
Scopus ID 85110617053
PubMed ID 34291054
Erfassungsdatum 2021-08-04
[➜Korrektur melden]