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Wettengel, J.M. ; Linden, B. ; Esser, K. ; Laue, M.* ; Burwitz, B.J.* ; Protzer, U.

Rapid and robust continuous purification of high-titer hepatitis B virus for in vitro and in vivo applications.

Viruses 13:1503 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Available treatments for hepatitis B can control the virus but are rarely curative. This led to a global initiative to design new curative therapies for the 257 million patients affected. Discovery and development of these new therapies is contingent upon functional in vitro and in vivo hepatitis B virus (HBV) infection models. However, low titer and impurity of conventional HBV stocks reduce significance of in vitro infections and moreover limit challenge doses in current in vivo models. Therefore, there is a critical need for a robust, simple and reproducible protocol to generate high-purity and high-titer infectious HBV stocks. Here, we outline a three-step protocol for continuous production of high-quality HBV stocks from supernatants of HBV-replicating cell lines. This purification process takes less than 6 h, yields to high-titer stocks (up to 1 × 1011 enveloped, DNA-containing HBV particles/mL each week), and is with minimal equipment easily adaptable to most laboratory settings.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Hbv For In Vitro And In Vivo Assays ; Hbv High-titer Virus Stocks ; Heparin-affinity Chromatography ; Hepatitis B Virus ; Sucrose-gradient Ultracentrifugation ; Virus Purification; Surface-antigen; Infection; Hbv; Particles; Cells; Ultracentrifugation; Hepatocytes; Expression; Liver
Sprache englisch
Veröffentlichungsjahr 2021
HGF-Berichtsjahr 2021
ISSN (print) / ISBN 1999-4915
e-ISSN 1999-4915
Zeitschrift Viruses
Quellenangaben Band: 13, Heft: 8, Seiten: , Artikelnummer: 1503 Supplement: ,
Verlag MDPI
Verlagsort St Alban-anlage 66, Ch-4052 Basel, Switzerland
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Virology (VIRO)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-502700-003
Förderungen German Research Foundation (Deutsche Forschungsgemeinschaft, DFG)
DOI 10.3390/v13081503
WOS ID WOS:000690291200001
Scopus ID 85111672979
PubMed ID 34452368
Erfassungsdatum 2021-09-17
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