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Fukumura, K.* ; Yoshimoto, R.* ; Sperotto, L. ; Kang, H.-S. ; Hirose, T.* ; Inoue, K.* ; Sattler, M. ; Mayeda, A.*

SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns.

Nat. Commun. 12:4910 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Human pre-mRNA introns vary in size from under fifty to over a million nucleotides. We searched for essential factors involved in the splicing of human short introns by screening siRNAs against 154 human nuclear proteins. The splicing activity was assayed with a model HNRNPH1 pre-mRNA containing short 56-nucleotide intron. We identify a known alternative splicing regulator SPF45 (RBM17) as a constitutive splicing factor that is required to splice out this 56-nt intron. Whole-transcriptome sequencing of SPF45-deficient cells reveals that SPF45 is essential in the efficient splicing of many short introns. To initiate the spliceosome assembly on a short intron with the truncated poly-pyrimidine tract, the U2AF-homology motif (UHM) of SPF45 competes out that of U2AF65 (U2AF2) for binding to the UHM-ligand motif (ULM) of the U2 snRNP protein SF3b155 (SF3B1). We propose that splicing in a distinct subset of human short introns depends on SPF45 but not U2AF heterodimer.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter U2af Homology Motif; Proteomic Analysis; Smn Complex; Sex-lethal; Rna; Recognition; Spliceosome; Protein; Mechanisms; Cells
Sprache englisch
Veröffentlichungsjahr 2021
HGF-Berichtsjahr 2021
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 12, Heft: 1, Seiten: , Artikelnummer: 4910 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503000-001
Förderungen Kobe University
DFG
JSPS
Nitto Foundation
Hori Sciences and Arts Foundation
Japan Society for the Promotion of Science (JSPS)
DOI 10.1038/s41467-021-24879-y
WOS ID WOS:000686635300008
Scopus ID 85112687593
PubMed ID 34389706
Erfassungsdatum 2021-09-21
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