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Singh, S.P.* ; Chawla, P.* ; Hnatiuk, A.* ; Kamel, M.* ; Silva, L.D.* ; Spanjaard, B.* ; Eski, S.E.* ; Janjuha, S.* ; Olivares-Chauvet, P.* ; Kayisoglu, O.* ; Rost, F.* ; Bläsche, J.* ; Kränkel, A.* ; Petzold, A.* ; Kurth, T.* ; Reinhardt, S.* ; Junker, J.P.* ; Ninov, N.

A single-cell atlas of de novo β-cell regeneration reveals the contribution of hybrid β/δ-cells to diabetes recovery in zebrafish.

Development 149:dev199853 (2022)
Verlagsversion Postprint Forschungsdaten DOI PMC
Open Access Green
Regeneration-competent species possess the ability to reverse the progression of severe diseases by restoring the function of the damaged tissue. However, the cellular dynamics underlying this capability remain unexplored. Here, we have used single-cell transcriptomics to map de novo β-cell regeneration during induction and recovery from diabetes in zebrafish. We show that the zebrafish has evolved two distinct types of somatostatin-producing δ-cells, which we term δ1- and δ2-cells. Moreover, we characterize a small population of glucose-responsive islet cells, which share the hormones and fate-determinants of both β- and δ1-cells. The transcriptomic analysis of β-cell regeneration reveals that β/δ hybrid cells provide a prominent source of insulin expression during diabetes recovery. Using in vivo calcium imaging and cell tracking, we further show that the hybrid cells form de novo and acquire glucose-responsiveness in the course of regeneration. The overexpression of dkk3, a gene enriched in hybrid cells, increases their formation in the absence of β-cell injury. Finally, interspecies comparison shows that plastic δ1-cells are partially related to PP cells in the human pancreas. Our work provides an atlas of β-cell regeneration and indicates that the rapid formation of glucose-responsive hybrid cells contributes to the resolution of diabetes in zebrafish.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Beta-cell ; Cell Fate ; Cell Plasticity ; Diabetes ; Gamma-cell ; Insulin ; Pancreas ; Regeneration ; Single Cell ; Zebrafish; Transgenic Zebrafish; Pancreatic-islets; Expression; Insulin; Fate; Differentiation; Generation; Progenitor; Conversion; Proteins
Sprache englisch
Veröffentlichungsjahr 2022
HGF-Berichtsjahr 2022
ISSN (print) / ISBN 0950-1991
e-ISSN 1477-9129
Zeitschrift Development / Company of Biologists
Quellenangaben Band: 149, Heft: 2, Seiten: , Artikelnummer: dev199853 Supplement: ,
Verlag Company of Biologists
Verlagsort Bidder Building, Station Rd, Histon, Cambridge Cb24 9lf, England
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502600-010
Förderungen International Research Training Group (IRTG 2251): 'Immunological and Cellular Strategies in Metabolic Disease'
Deutsche Forschungsgemeinschaft
Deutsches Zentrum fur Diabetesforschung (DZD)
Center for Regenerative Therapies Dresden at Technische Universitat Dresden
Fonds de la Recherche Scientifique-FNRS
European Research Council starting grant
DOI 10.1242/dev.199853
WOS ID WOS:000759115300013
Scopus ID 85124446327
PubMed ID 35088828
Erfassungsdatum 2022-06-23
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