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Beer, S. ; Wange, L.E.* ; Zhang, X. ; Kuklik-Roos, C. ; Enard, W.* ; Hammerschmidt, W. ; Scialdone, A. ; Kempkes, B.

EBNA2-EBF1 complexes promote MYC expression and metabolic processes driving S-phase progression of Epstein-Barr virus-infected B cells.

Proc. Natl. Acad. Sci. U.S.A. 119:e2200512119 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Epstein-Barr virus (EBV) is a human tumor virus which preferentially infects resting human B cells. Upon infection in vitro, EBV activates and immortalizes these cells. The viral latent protein EBV nuclear antigen 2 (EBNA2) is essential for B cell activation and immortalization; it targets and binds the cellular and ubiquitously expressed DNA-binding protein CBF1, thereby transactivating a plethora of viral and cellular genes. In addition, EBNA2 uses its N-terminal dimerization (END) domain to bind early B cell factor 1 (EBF1), a pioneer transcription factor specifying the B cell lineage. We found that EBNA2 exploits EBF1 to support key metabolic processes and to foster cell cycle progression of infected B cells in their first cell cycles upon activation. The α1-helix within the END domain was found to promote EBF1 binding. EBV mutants lacking the α1-helix in EBNA2 can infect and activate B cells efficiently, but activated cells fail to complete the early S phase of their initial cell cycle. Expression of MYC, target genes of MYC and E2F, as well as multiple metabolic processes linked to cell cycle progression are impaired in EBVΔα1-infected B cells. Our findings indicate that EBF1 controls B cell activation via EBNA2 and, thus, has a critical role in regulating the cell cycle of EBV-infected B cells. This is a function of EBF1 going beyond its well-known contribution to B cell lineage specification.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter B Cell Activation ; Epstein-barr Virus ; Myc Expression ; Rna Sequencing ; Transcription Factor; Rbp-j-kappa; Nuclear Antigen-2; Binding-protein; C-myc; Latent Membrane-protein-1; Transcription Factors; Proliferation; Ebf1; Lymphocytes; Genes
Sprache englisch
Veröffentlichungsjahr 2022
HGF-Berichtsjahr 2022
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Zeitschrift Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Band: 119, Heft: 30, Seiten: , Artikelnummer: e2200512119 Supplement: ,
Verlag National Academy of Sciences
Verlagsort 2101 Constitution Ave Nw, Washington, Dc 20418 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Gene Vector (AGV)
Institute of Epigenetics and Stem Cells (IES)
Institute of Functional Epigenetics (IFE)
Institute of Computational Biology (ICB)
POF Topic(s) 30203 - Molecular Targets and Therapies
30204 - Cell Programming and Repair
30205 - Bioengineering and Digital Health
Forschungsfeld(er) Immune Response and Infection
Stem Cell and Neuroscience
Helmholtz Diabetes Center
Enabling and Novel Technologies
PSP-Element(e) G-501500-002
G-501500-001
G-501500-003
G-506290-001
G-502800-001
G-503800-001
Förderungen Deutsche Forschungsgemeinschaft (DFG)
DOI 10.1073/pnas.2200512119
WOS ID 001025742800009
Scopus ID 85134738091
PubMed ID 35857872
Erfassungsdatum 2022-11-03
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