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Sigmund, F. ; Berezin, O. ; Beliakova, S. ; Magerl, B. ; Drawitsch, M.* ; Piovesan, A. ; Gonçalves, F. ; Bodea, S.V. ; Winkler, S. ; Bousraou, Z. ; Grosshauser, M. ; Samara, E.* ; Pujol-Martí, J.* ; Schädler, S.* ; So, C.* ; Irsen, S.* ; Walch, A.K. ; Kofler, F. ; Piraud, M. ; Kornfeld, J.* ; Briggman, K.* ; Westmeyer, G.G.

Genetically encoded barcodes for correlative volume electron microscopy.

Nat. Biotechnol. 41, 1734–1745 (2023)
Verlagsversion DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
While genetically encoded reporters are common for fluorescence microscopy, equivalent multiplexable gene reporters for electron microscopy (EM) are still scarce. Here, by installing a variable number of fixation-stable metal-interacting moieties in the lumen of encapsulin nanocompartments of different sizes, we developed a suite of spherically symmetric and concentric barcodes (EMcapsulins) that are readable by standard EM techniques. Six classes of EMcapsulins could be automatically segmented and differentiated. The coding capacity was further increased by arranging several EMcapsulins into distinct patterns via a set of rigid spacers of variable length. Fluorescent EMcapsulins were expressed to monitor subcellular structures in light and EM. Neuronal expression in Drosophila and mouse brains enabled the automatic identification of genetically defined cells in EM. EMcapsulins are compatible with transmission EM, scanning EM and focused ion beam scanning EM. The expandable palette of genetically controlled EM-readable barcodes can augment anatomical EM images with multiplexed gene expression maps.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Metallothionein; Protein; Tag; Binding; Pb(ii); Cells
Sprache englisch
Veröffentlichungsjahr 2023
HGF-Berichtsjahr 2023
ISSN (print) / ISBN 1087-0156
e-ISSN 1546-1696
Zeitschrift Nature Biotechnology
Quellenangaben Band: 41, Heft: , Seiten: 1734–1745 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed
Institut(e) Insitute of Synthetic Biomedicine (ISBM)
Research Unit Analytical Pathology (AAP)
Helmholtz Artifical Intelligence Cooperation Unit (HAICU)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-509300-001
G-500390-001
G-530001-001
DOI 10.1038/s41587-023-01713-y
WOS ID 000970910100001
Scopus ID 85153038499
PubMed ID 37069313
Erfassungsdatum 2023-10-06
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