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Römisch-Margl, W. ; Prehn, C. ; Bogumil, R.* ; Röhring, C.* ; Suhre, K. ; Adamski, J.

Procedure for tissue sample preparation and metabolite extraction for high-throughput targeted metabolomics.

Metabolomics 8, 133-142 (2012)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Reproducible quantification of metabolites in tissue samples is of high importance for characterization of animal models and identification of metabolic changes that occur in different tissue types in specific diseases. However, the extraction of metabolites from tissue is often the most labor-intensive and error-prone step in metabolomics studies. Here, we report the development of a standardized high-throughput method for rapid and reproducible extraction of metabolites from multiple tissue samples from different organs of several species. The method involves a bead-based homogenizer in combination with a simple extraction protocol and is compatible with state-ofthe-art metabolomics kit technology for quantitative and targeted flow injection tandem mass spectrometry. We analyzed different extraction solvents for both reproducibility as well as suppression effects for a range of different animal tissue types including liver, kidney, muscle, brain, and fat tissue from mouse and bovine. In this study, we show that for most metabolites a simple methanolic extraction is best suited for reliable results. An additional extraction step with phosphate buffer can be used to improve the extraction yields for a few more polar metabolites. We provide a verified tissue extraction setup to be used with different indications. Our results demonstrate that this high-throughput procedure provides a basis for metabolomic assays with a wide spectrum of metabolites. The developed method can be used for tissue extraction setup for different indications like studies of metabolic syndrome, obesity, diabetes or cardiovascular disorders and nutrient transformation in livestock.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Mass spectrometry; Tissue extraction; High-throughput; Quantification; Complex diseases; Food quality
Sprache englisch
Veröffentlichungsjahr 2012
Prepublished im Jahr 2011
HGF-Berichtsjahr 2011
ISSN (print) / ISBN 1573-3882
e-ISSN 1573-3890
Zeitschrift Metabolomics
Quellenangaben Band: 8, Heft: 1, Seiten: 133-142 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Bioinformatics and Systems Biology (IBIS)
Molekulare Endokrinologie und Metabolismus (MEM)
POF Topic(s) 30505 - New Technologies for Biomedical Discoveries
30201 - Metabolic Health
Forschungsfeld(er) Enabling and Novel Technologies
Genetics and Epidemiology
PSP-Element(e) G-503700-001
G-505600-001
DOI 10.1007/s11306-011-0293-4
Scopus ID 84855875727
Erfassungsdatum 2011-12-31
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