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Abu Laban, N.M.F.H. ; Selesi, D. ; Jobelius, C.* ; Meckenstock, R.U.

Anaerobic benzene degradation by Gram-positive sulfate-reducing bacteria.

FEMS Microbiol. Ecol. 68, 300-311 (2009)
Verlagsversion DOI PMC
Open Access Gold
Despite its high chemical stability, benzene is known to be biodegradable with various electron acceptors under anaerobic conditions. However, our understanding of the initial activation reaction and the responsible prokaryotes is limited. In the present study, we enriched a bacterial culture that oxidizes benzene to carbon dioxide under sulfate-reducing conditions. Community analysis using terminal restriction fragment length polymorphism, 16S rRNA gene sequencing and FISH revealed 95% dominance of one phylotype that is affiliated to the Gram-positive bacterial genus Pelotomaculum showing that sulfate-reducing Gram-positive bacteria are involved in anaerobic benzene degradation. In order to get indications of the initial activation mechanism, we tested the substrate utilization, performed cometabolism tests and screened for putative metabolites. Phenol, toluene, and benzoate could not be utilized as alternative carbon sources by the benzene-degrading culture. Cometabolic degradation experiments resulted in retarded rates of benzene degradation in the presence of phenol whereas toluene had no effect on benzene metabolism. Phenol, 2-hydroxybenzoate, 4-hydroxybenzoate, and benzoate were identified as putative metabolites in the enrichment culture. However, hydroxylated aromatics were shown to be formed abiotically. Thus, the finding of benzoate as an intermediate compound supports a direct carboxylation of benzene as the initial activation mechanism but additional reactions leading to its formation cannot be excluded definitely.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter benzene; sulfate reduction; Gram-positive bacteria; petroleum-contaminated aquifer; molecular characterization; sp-nov; enrichment culture; microbial communities; oxidizing bacterium; nitrate reduction; o-xylene; desulfotomaculum; benzoate
Sprache englisch
Veröffentlichungsjahr 2009
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0168-6496
e-ISSN 1574-6941
Zeitschrift FEMS Microbiology Ecology
Quellenangaben Band: 68, Heft: 3, Seiten: 300-311 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Oxford
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Groundwater Ecology (IGOE)
POF Topic(s) 20403 - Sustainable Water Management
Forschungsfeld(er) Environmental Sciences
PSP-Element(e) G-504300-004
PubMed ID 19416354
DOI 10.1111/j.1574-6941.2009.00672.x
Scopus ID 65549142775
Erfassungsdatum 2009-07-09
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